NIPBL Antibody - BSA Free

Western Blot: NIPBL Antibody [NB100-93320] - Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NIPBL antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunoprecipitation: NIPBL Antibody [NB100-93320] - Detection of human NIPBL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NIPBL antibody NB100-93320 (lot 4) used for IP at 3 ug per reaction. NIPBL was also immunoprecipitated by a previous lot of this antibody (lot 3) and rabbit anti-NIPBL antibody NB100-93319. For blotting immunoprecipitated NIPBL, NB100-93320 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

NIPBL Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1025 and 1075 of human Nipped-B-like using the numbering given in entry NP_597677.2 (GeneID 25836).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NIPBL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-6 ug/mg lysate
  • Western Blot 1:2000 - 1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NIPBL Antibody - BSA Free

• CDLS1
• delangin
• DKFZp434L1319
• FLJ11203
• FLJ12597
• FLJ13354
• FLJ13648
• FLJ44854
• IDN3-B
• IDN3CDLS
• Nipped-B homolog (Drosophila)
• nipped-B-like protein
• SCC2 homolog
• Scc2
• sister chromatid cohesion 2 homolog

Background

This gene encodes the homolog of the Drosophila melanogaster Nipped-B gene product and fungal Scc2-type sister chromatid cohesion proteins. The Drosophila protein facilitates enhancer-promoter communication of remote enhancers and plays a role in developmental regulation. It is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. The human protein has a bipartite nuclear targeting sequence and a putative HEAT repeat. Condensins, cohesins and other complexes with chromosome-related functions also contain HEAT repeats. Mutations in this gene result in Cornelia de Lange syndrome, a disorder characterized by dysmorphic facial features, growth delay, limb reduction defects, and mental retardation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NIPBL Antibody (NB100-93320)(1)

Publication using NB100-93320

• Mazzola M, Deflorian G, Pezzotta A et al. NIPBL: a new player in myeloid cells differentiation Haematologica 2019-01-10 [PMID: 30630974] (WB, Human)

Bioinformatics

• Gene Symbol: NIPBL
• Entrez:
  • 25836(Human)
• Uniprot:
  • NP_597677.2(Human)