| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85110-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | NBP3-47340AF488 | |||
| Alexa Fluor 647 | NBP3-47340AF647 | |||
| Alexa Fluor 700 | NBP3-47340AF700 | |||
| Allophycocyanin | NBP3-47340APC | |||
| CoraFluor 1 | NBP3-47340CL1 | |||
| PE | NBP3-47340PE | |||
| PerCP | NBP3-47340PCP | |||
| mFluor Violet 500 SE | NBP3-47340MFV500 | |||
![Immunocytochemistry/Immunofluorescence: NIPBL Antibody [NBP1-85110] - Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/NIPBL-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85110-img0007.jpg "Immunocytochemistry/Immunofluorescence: NIPBL Antibody [NBP1-85110] - Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-85110] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/NIPBL-Antibody-Immunohistochemistry-Paraffin-NBP1-85110-img0008.jpg)
![Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-85110] -Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/antibody/nbp1-85110_rabbit-polyclonal-nipbl-antibody-immunohistochemistry-paraffin-952024115457..jpg)
![Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-85110] -Staining of human skin shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/antibody/nbp1-85110_rabbit-polyclonal-nipbl-antibody-immunohistochemistry-paraffin-952024115556..jpg)
![Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-85110] - Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-85110_rabbit-polyclonal-nipbl-antibody-immunohistochemistry-paraffin-952024115657..jpg)
![Immunocytochemistry/Immunofluorescence: NIPBL Antibody [NBP1-52168] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear and cytoplasmic staining. The nuclear stai](http://images.novusbio.com/fullsize/NIPBL-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-52168-img0003.jpg "Immunocytochemistry/Immunofluorescence: NIPBL Antibody [NBP1-52168] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear and cytoplasmic staining. The nuclear stai")
![Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-52168] - (2ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/NIPBL-Antibody-Immunohistochemistry-Paraffin-NBP1-52168-img0004.jpg)
![Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-52168] - Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.](http://images.novusbio.com/fullsize/NIPBL-Antibody-Immunohistochemistry-Paraffin-NBP1-52168-img0001.jpg)
![Western Blot: NIPBL Antibody (3B9) [H00025836-M01] - Detection against Immunogen (37.73 KDa) .](http://images.novusbio.com/fullsize/NIPBL-Antibody-3B9-Western-Blot-H00025836-M01-img0003.jpg "Western Blot: NIPBL Antibody (3B9) [H00025836-M01] - Detection against Immunogen (37.73 KDa) .")
![Immunocytochemistry/Immunofluorescence: NIPBL Antibody (3B9) [H00025836-M01] - Analysis of monoclonal antibody to NIPBL on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NIPBL-Antibody-3B9-Immunocytochemistry-Immunofluorescence-H00025836-M01-img0002.jpg)
![Western Blot: NIPBL Antibody [NBP1-77974] - Detection of a band ~300 kDa corresponding to IDN3 (arrowhead) in human kidney (lane 1) and human heart (lane 2) whole cell tissue extracts. Approximately 20ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Use HRP conjugated Rb-a-Goat IgG [H&L] MXHu for detection.](http://images.novusbio.com/fullsize/NIPBL-Antibody-Western-Blot-NBP1-77974-img0002.jpg "Western Blot: NIPBL Antibody [NBP1-77974] - Detection of a band ~300 kDa corresponding to IDN3 (arrowhead) in human kidney (lane 1) and human heart (lane 2) whole cell tissue extracts. Approximately 20ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Use HRP conjugated Rb-a-Goat IgG [H&L] MXHu for detection.")
![Western Blot: NIPBL Antibody [NBP1-77974] - Shows detection of bands at ~315 kDa and ~125 kDa corresponding to isoforms of IDN3 (arrow-heads) in mouse heart whole cell tissue extract. Approximately 35 ug of lysate was separated on a 4-8% gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Reaction occurred overnight at 4h followed by washes and reaction with a 1:20,000 dilution of IRDye (TM) 800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.](http://images.novusbio.com/fullsize/NIPBL-Antibody-Western-Blot-NBP1-77974-img0001.jpg)
![Western Blot: NIPBL Antibody [NB100-93320] - Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NIPBL antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/NIPBL-Antibody-Western-Blot-NB100-93320-img0006.jpg "Western Blot: NIPBL Antibody [NB100-93320] - Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NIPBL antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: NIPBL Antibody [NB100-93320] - Detection of human NIPBL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NIPBL antibody NB100-93320 (lot 4) used for IP at 3 ug per reaction. NIPBL was also immunoprecipitated by a previous lot of this antibody (lot 3) and rabbit anti-NIPBL antibody NB100-93319. For blotting immunoprecipitated NIPBL, NB100-93320 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/NIPBL-Antibody-Immunoprecipitation-NB100-93320-img0007.jpg)
![Western Blot: NIPBL Antibody - BSA Free [NBP3-47340] - HeLa cells were subjected to SDS PAGE followed by western blot with (NIPBL antibody) at dilution of 1:800](http://images.novusbio.com/fullsize/nbp3-47340_rabbit-nipbl-pab-bsa-free-28420251231257.jpg "Western Blot: NIPBL Antibody - BSA Free [NBP3-47340] - HeLa cells were subjected to SDS PAGE followed by western blot with (NIPBL antibody) at dilution of 1:800")