NIK/MAP3K14 Antibody - Azide and BSA Free

Western Blot: NIK/MAP3K14 Antibody [NB100-56367] - Analysis of NIK/MAP3K14 in (A) untransfected and (B) transfected 293 cells using this antibody.

Immunocytochemistry/Immunofluorescence: NIK/MAP3K14 Antibody [NB100-56367] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with NIK/MAP3K14 (NB100-56367) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with Dylight 550 (Red) at a 1:500 dilution. Nuclei were detected with DAPI (Blue) at 2.0ug/ml in 1X PBS. Cells were imaged using a 40X objective.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

NIK/MAP3K14 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit NIK/MAP3K14 Antibody - Azide and BSA Free (NB100-56367) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 687-703 of human NIK.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAP3K14
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1-3 ug/ml
• Control:

  NIK/MAP3K14 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for NIK/MAP3K14 Antibody - Azide and BSA Free

• EC 2.7.11.25
• FTDCR1B
• HS
• HSNIK
• HSNIKserine/threonine protein-kinase
• MAP3K14
• mitogen-activated protein kinase kinase kinase 14
• NIK
• NIKNF-kappa-beta-inducing kinase
• Serine/threonine-protein kinase NIK

Background

NIK (NF-kB-inducing Kinase) expression leads to IkB kinase activation and induces nuclear translocation of NF-kB. It is involved in regulation of apoptosis, tumorigenesis, inflammation and various autoimmune diseases. In PC12 cells it induces differentiation and prevents apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP3K14
• Entrez:
  • 9020(Human)
• Uniprot:
  • Q99558()