NIFK Antibody

Images

 
Western Blot: NIFK Antibody [NB100-56660] - Western blot analysis of NIFK in 30 ugs of HeLa cell lysate using NB100-56660 at 1:1000.
Immunohistochemistry: NIFK Antibody [NB100-56660] - Staining in adipocytes of human carcinomatus breast tissue using NB100-56660 at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-Fr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

NIFK Antibody Summary

Immunogen
This antibody was developed against synthetic peptides corresponding to amino acids 144-160 and 276-293 of human NIFK. Amino acids 144-160 share a 40% homology with mouse NIFK.
Specificity
In HeLa, a 34 kDa band is observed. IHC: Formalin-fixed paraffin-embedded breast carcinoma.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MKI67IP
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
Positive Control
NIFK Lysate (NBL1-13120)
HeLa Lysate (NB800-PC1)

Reactivity Notes

Cross reacts with Human. Predicted to cross react with Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for NIFK Antibody

  • hNIFK
  • MKI67 (FHA domain) interacting nucleolar phosphoprotein
  • MKI67 FHA domain-interacting nucleolar phosphoprotein
  • NIFKNOPP34
  • Nopp34
  • Nucleolar phosphoprotein Nopp34
  • Nucleolar protein interacting with the FHA domain of pKI-67

Background

NIFK is a nucleolar protein that interacts with Ki67, a protein strongly associated with, and a specific marker of, cell proliferation. The marsupial counterpart of human NIFK was recently identified and suggests that NIFK may be involved in the organization of higher order chromatin structure. NIFK was found to interact with the forkhead associated domain of Ki67 in mitotic cells and the interaction is thought to be phosphorylation-dependent.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MKI67IP
Entrez
Uniprot