NHP2L1 Antibody

Images

 
Western Blot: NHP2L1 Antibody [NBP1-32732] - A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract ...read more
Immunocytochemistry/ Immunofluorescence: NHP2L1 Antibody [NBP1-32732] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: NHP2L1 Antibody [NBP1-32732] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
Western Blot: NHP2L1 Antibody [NBP1-32732] - Sample (30 ug of whole cell lysate)A: HeLaB: Hep G2 12% SDS PAGE, antibody diluted at 1:1000.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

NHP2L1 Antibody Summary

Immunogen
Full length human NHP2-like protein 1 Recombinant protein.
Localization
Nucleus, nucleolus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NHP2L1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-32732 in the following applications:

  • IP
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NHP2L1 Antibody

  • [U4/U6.U5] tri-snRNP 15.5 kD RNA binding protein
  • 15.5K
  • FA1
  • FA-1
  • High mobility group-like nuclear protein 2 homolog 1
  • hSNU13
  • NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae)
  • NHP2-like protein 1
  • NHPX
  • non-histone chromosome protein 2 (S. cerevisiae)-like 1
  • non-histone chromosome protein 2-like 1
  • OTK27
  • small nuclear ribonucleoprotein 15.5kDa (U4/U6.U5)
  • SNRNP15-5
  • SNU13 homolog
  • SNU13
  • SPAG12
  • sperm specific antigen 1
  • SSFA1
  • U4/U6.U5 tri-snRNP 15.5 kDa protein

Background

Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non-histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri-snRNP. It binds to the 5' stem-loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NHP2L1 Antibody (NBP1-32732)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IP.


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(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NHP2L1 Antibody (NBP1-32732). (Showing 1 - 1 of 1 FAQ).

  1. Have you guys tested the NHP2L1 antibodies (ANTI-15.5K ANTIBODY) (cat# NBP1-32732) for IP experiments? Can it be used for whole cell extract 15.5K immunoprecipitation?
    • This is not an application we have tested, and therefore we cannot say for certain whether this application will work. Due to the fact that it does work in WB there is a good chance the antibody will also work in IP. Even though Novus cannot guarantee it, we can recommend our Innovators Reward Program. Our Innovator's Reward is offered to reward researchers for testing new species and applications with our products. If the testing for this antibody is performed on an untested species and/or application, and the results are shared, then the antibody is eligible for the Innovator's Reward. Under this program, Novus will provide you a credit of equal or lesser value on a future product in exchange for your new data in the form of an online review. The great thing is you are eligible even if the antibody doesn't work because the data is still useful to us.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NHP2L1
Entrez
Uniprot