Western Blot: NHP2L1 Antibody [NBP1-32732] - A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract ...read more
Immunocytochemistry/ Immunofluorescence: NHP2L1 Antibody [NBP1-32732] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: NHP2L1 Antibody [NBP1-32732] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
Western Blot: NHP2L1 Antibody [NBP1-32732] - Sample (30 ug of whole cell lysate)A: HeLaB: Hep G2 12% SDS PAGE, antibody diluted at 1:1000.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7).
Immunogen affinity purified
For IHC-P: Suggested antigen retrieval using heat mediated 10mM Citrate buffer(PH6.0) or Tris-EDTA buffer ( PH8.0).
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NBP1-32732 in the following applications:
High mobility group-like nuclear protein 2 homolog 1
NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae)
NHP2-like protein 1
non-histone chromosome protein 2 (S. cerevisiae)-like 1
non-histone chromosome protein 2-like 1
small nuclear ribonucleoprotein 15.5kDa (U4/U6.U5)
sperm specific antigen 1
U4/U6.U5 tri-snRNP 15.5 kDa protein
Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non-histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri-snRNP. It binds to the 5' stem-loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for NHP2L1 Antibody (NBP1-32732). (Showing 1 - 1 of 1 FAQ).
Have you guys tested the NHP2L1 antibodies (ANTI-15.5K ANTIBODY) (cat# NBP1-32732) for IP experiments? Can it be used for whole cell extract 15.5K immunoprecipitation?
This is not an application we have tested, and therefore we cannot say for certain whether this application will work. Due to the fact that it does work in WB there is a good chance the antibody will also work in IP. Even though Novus cannot guarantee it, we can recommend our Innovators Reward Program. Our Innovator's Reward is offered to reward researchers for testing new species and applications with our products. If the testing for this antibody is performed on an untested species and/or application, and the results are shared, then the antibody is eligible for the Innovator's Reward. Under this program, Novus will provide you a credit of equal or lesser value on a future product in exchange for your new data in the form of an online review. The great thing is you are eligible even if the antibody doesn't work because the data is still useful to us.