NOP56 Antibody (CL2603) - BSA Free

Western Blot: NOP56 Antibody (CL2603) [NBP2-36778] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NOP56 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778] - Staining in MCF7 cell line with Anti-NOP56 monoclonal antibody, showing nucleoli-specific staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human breast cancer shows strong nucleolar positivity in tumor cells.

Western Blot: NOP56 Antibody (CL2603) [NBP2-36778] - Lane 1: Marker [kDa]. Lane 2:Human cell line U-251 MG

Immunocytochemistry/Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778] - Staining in HeLa cell line showing nucleoli-specific staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human cell line A431 showing nucleoli-specific staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778] - Staining in U2OS cell line with Anti-NOP56 monoclonal antibody, showing nucleoli-specific staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778] - Staining in U251 cell line showing nucleoli-specific staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human prostate cancer shows strong nucleolar positivity in glandular cells.

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human fallopian tube shows strong nucleolar immunoreactivity in glandular and connective tissue cell

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human kidney shows strong nucleolar positivity in renal tubules.

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human cervix shows strong nucleolar immunoreactivity in the epithelial cells.

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human liver shows strong nucleolar positivity in hepatocytes.

NOP56 (CL2603) was detected in immersion fixed A431 human skin carcinoma cell line using Mouse anti-NOP56 (CL2603) Protein A Purified Monoclonal Antibody conjugated to Alexa Fluor® 488 (Catalog # NBP2-36778) (green) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clone: CL2603
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

NOP56 Antibody (CL2603) - BSA Free Summary

• Description: Novus Biologicals Mouse NOP56 Antibody (CL2603) - BSA Free (NBP2-36778) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from O00567, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NOP56
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Knockdown Validated
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  NOP56 Recombinant Protein Antigen (NBP2-36778PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for NOP56 Antibody (CL2603) - BSA Free

• NOL5A
• NOP56 ribonucleoprotein homolog (yeast)
• nucleolar protein 56
• nucleolar protein 5A (56kD with KKE/D repeat)
• nucleolar protein 5A (56kDa with KKE/D repeat)
• Nucleolar protein 5A

Background

Nop56p is a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Nop56p is required for assembly of the 60S ribosomal subunit and is involved in pre-rRNA processing. The protein encoded by this gene is similar in sequence to Nop56p and is also found in the nucleolus. Multiple transcript variants encoding several different isoforms have been found for this gene, but the full-length nature of most of them have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NOP56
• Uniprot:
  • O00567(Human)