PRPF31 Antibody

Images

 
Western Blot: PRPF31 Antibody [NB100-55409] - Staining (1ug/ml) of A431 (A), HeLa (B) and HEK293 (C) nuclear cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: PRPF31 Antibody [NB100-55409] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by ...read more
Flow Cytometry: PRPF31 Antibody [NB100-55409] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more
Western Blot: PRPF31 Antibody [NB100-55409] - Staining (1ug/ml) of NIH3T3 nuclear cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: PRPF31 Antibody [NB100-55409] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, Flow, ICC/IF, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PRPF31 Antibody Summary

Immunogen
Peptide with sequence KELGNSLDKCKNNEN corresponding to internal region according to NP_056444.3.
Epitope
KELGNSLDKCKNNEN
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PRPF31
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in A431, HeLa, HEK293 and NIH3T3 lysates (calculated MW of 55.4 kDa band according to NP_056444.3).
Control
PRPF31 Overexpression Lysate
HeLa Whole Cell Lysate
Publications
Read Publication using NB100-55409.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PRPF31 Antibody

  • DKFZp566J153
  • hPrp31
  • NY-BR-99
  • Pre-mRNA-processing factor 31
  • Protein 61K
  • PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae)
  • PRP31 pre-mRNA processing factor 31 homolog (yeast)
  • PRP31RP11
  • Serologically defined breast cancer antigen NY-BR-99
  • U4/U6 small nuclear ribonucleoprotein Prp31
  • U4/U6 snRNP 61 kDa protein

Background

Retinitis pigmentosa (RP) is a clinically and genetically heterogeneous group of retinal dystrophies characterized by a progressive degeneration of photoreceptors, eventually resulting in severe visual impairment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PRPF31 Antibody (NB100-55409)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PRPF31 Antibody (NB100-55409) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Calculators

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Bioinformatics

Gene Symbol PRPF31
Entrez
Uniprot