This antibody has been successfully used in Western blot, Immunohistochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~50 kDa protein representing EBP50 from T84 cell extract. Immunohistochemical staining of EBP50 in mouse airway results in staining of the epithelium. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 14625387).
Rat reactivity reported in scientific literature (PMID: 21761111). Canine reactivity reported in scientific literature (PMID: 21444686).
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
PBS, 1 mg/mL BSA
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for NHERF-1 Antibody
ezrin-radixin-moesin binding phosphoprotein-50
Ezrin-radixin-moesin-binding phosphoprotein 50
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
Na+/H+ exchange regulatory co-factor
NHERF1solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1
Regulatory cofactor of Na(+)/H(+) exchanger
sodium/hydrogen exchanger regulatory factor 1
Sodium-hydrogen exchanger regulatory factor 1
solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 1
solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
Solute carrier family 9 isoform A3 regulatory factor 1
EBP 50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50 kDa] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP 50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes.
EBP 50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin. Through these interactions, EBP 50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins. Immunohistochemical studies have shown that EBP 50 is found exclusively at the apical membranes of epithelial cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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