NHERF-1 Antibody

Western Blot: NHERF-1 Antibody [NB300-536] - Analysis of recombinant human EBP50.

Immunocytochemistry/Immunofluorescence: NHERF-1 Antibody [NB300-536] - Phosphatase and NHERF1 assembly at the immunologic synapse. PTEN, NHERF-1, and PHLLP subcellular localization in relation to the central supramolecular activation complex (indicated by CD3 staining) and peripheral supramolecular activation complex (indicated by ICAM-1 staining) was analyzed by using TIRF microscopy. Differences were analyzed by using the Mann-Whitney U test. Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0091674916304973) licensed under a CC-BY license.

Immunohistochemistry: NHERF-1 Antibody [NB300-536] - Immunohistochemical staining of NERF-1 / EBP50 in mouse airway.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

NHERF-1 Antibody Summary

• Description: Novus Biologicals Rabbit NHERF-1 Antibody (NB300-536) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-NHERF-1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Bacterially expressed, His-tagged human EBP50.
• Specificity: Detects EBP50 from mouse tissues, human T84 and Calu3 cells as well as recombinant human protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NHERF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2 ug/mL
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 10 ug/mL
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1 ug/mL
• Application Notes: This antibody has been successfully used in Western blot, Immunohistochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~50 kDa protein representing EBP50 from T84 cell extract. Immunohistochemical staining of EBP50 in mouse airway results in staining of the epithelium. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 14625387).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 21761111). Canine reactivity reported in scientific literature (PMID: 21444686).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1 mg/mL BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NHERF-1 Antibody

• EBP50NHERF-1
• ezrin-radixin-moesin binding phosphoprotein-50
• Ezrin-radixin-moesin-binding phosphoprotein 50
• Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• Na+/H+ exchange regulatory co-factor
• NHERF1solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1
• NHERFNPHLOP2
• Regulatory cofactor of Na(+)/H(+) exchanger
• sodium/hydrogen exchanger regulatory factor 1
• Sodium-hydrogen exchanger regulatory factor 1
• solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 1
• solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
• Solute carrier family 9 isoform A3 regulatory factor 1

Background

EBP 50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50 kDa] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP 50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes.

EBP 50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin. Through these interactions, EBP 50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins. Immunohistochemical studies have shown that EBP 50 is found exclusively at the apical membranes of epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NHERF-1 Antibody (NB300-536)(4)

Publications using NB300-536

• Chen HH, Handel N, Ngeow J et al. Immune dysregulation in patients with PTEN hamartoma tumor syndrome: Analysis of FOXP3 regulatory T cells. J. Allergy Clin. Immunol. 2017-02-01 [PMID: 27477328]
• Ji M, Fan D, Yuan L et al. EBP50 inhibits pancreatic cancer cell growth and invasion by targeting the beta-catenin/E-cadherin pathway. Exp Ther Med 2015-10-01 [PMID: 26622484] (IF/IHC)
• Huang T, You Y, Spoor MS, Richer EJ, Kudva VV, Paige RC, Seiler MP, Liebler JM, Zabner J, Plopper CG, Brody SL. Foxj1 is required for apical localization of ezrin in airway epithelial cells. J Cell Sci;116(Pt 24):4935-45. 2003-12-15 [PMID: 14625387] (ICC/IF)
• Hartmann C, Thüring E, Michels B, et al. A novel adhesive complex at the base of intestinal microcilli bioRxiv 2021-01-28

Bioinformatics

• Gene Symbol: NHERF1
• Entrez:
  • 9368(Human)
  • 26941(Mouse)
• Uniprot:
  • O14745(Human)