NHERF-1 Antibody

Images

 
Western Blot: NHERF-1 Antibody [NB300-536] - Analysis of recombinant human EBP50.
Immunohistochemistry: NHERF-1 Antibody [NB300-536] - Immunohistochemical staining of NERF-1 / EBP50 in mouse airway.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

NHERF-1 Antibody Summary

Immunogen
Bacterially expressed, His-tagged human EBP50.
Specificity
Detects EBP50 from mouse tissues, human T84 and Calu3 cells as well as recombinant human protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC9A3R1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunocytochemistry/Immunofluorescence 2 ug/mL
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 10 ug/mL
  • Immunoprecipitation 1:10 - 1:500
Application Notes
This antibody has been successfully used in Western blot, Immunohistochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~50 kDa protein representing EBP50 from T84 cell extract. Immunohistochemical staining of EBP50 in mouse airway results in staining of the epithelium. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 14625387).
Control
NHERF-1 Knockout HeLa Cell Lysate
Publications
Read Publications using NB300-536.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 21761111). Canine reactivity reported in scientific literature (PMID: 21444686).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 1 mg/mL BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NHERF-1 Antibody

  • EBP50NHERF-1
  • ezrin-radixin-moesin binding phosphoprotein-50
  • Ezrin-radixin-moesin-binding phosphoprotein 50
  • Na(+)/H(+) exchange regulatory cofactor NHE-RF1
  • Na+/H+ exchange regulatory co-factor
  • NHERF1solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1
  • NHERFNPHLOP2
  • Regulatory cofactor of Na(+)/H(+) exchanger
  • sodium/hydrogen exchanger regulatory factor 1
  • Sodium-hydrogen exchanger regulatory factor 1
  • solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 1
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
  • Solute carrier family 9 isoform A3 regulatory factor 1

Background

EBP 50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50 kDa] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP 50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes.

EBP 50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin. Through these interactions, EBP 50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins. Immunohistochemical studies have shown that EBP 50 is found exclusively at the apical membranes of epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NHERF-1 Antibody (NB300-536)(3)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol SLC9A3R1
Entrez
Uniprot