NGFR/TNFRSF16/p75NTR Antibody (74902) [Unconjugated]

NGF R/TNFRSF16 was detected in immersion fixed canine mesenchymal stem cells using Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

NGF R/TNFRSF16 was detected in immersion fixed human mesenchymal stem cells using Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

NGF R/TNFRSF16 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 25 µg/mL Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NGF R/TNFRSF16 at approximately 65 kDa (as indicated). GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

SH‑SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Ca
• Applications: WB, Flow, IHC, CyTOF-ready, ICC/IF
• Clone: 74902
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NGFR/TNFRSF16/p75NTR Antibody (74902) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NGF R
  Lys29-Asn250
  Accession # P08138
• Specificity: Detects human NGF R in direct ELISAs and Western blots. In direct ELISAs, no crossreactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD40, rhBAFF R, rhCD30, rhDR3, rhDR6, rhEDAR, rhFas, rhHVEM, rhGITR, rhLTR B, recominant mouse (rm) NGF R, rhOPG, rmOX40, rhRANK, rhTAJ, rhTNF RI or rhTNF RII is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NGFR
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NGFR/TNFRSF16/p75NTR Antibody (74902) [Unconjugated]

• CD271 antigen
• CD271
• Gp80-LNGFR
• member 16
• nerve growth factor receptor
• NGF R
• NGF receptor
• NGFR
• p75 ICD
• p75 NTR
• TNFRSF16
• TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16)
• tumor necrosis factor receptor superfamily member 16

Background

NGF R is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family (1) and has been designated TNFRSF16. This receptor is also known as p75 NTR (neurotrophin receptor) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including Brain-Derived Neurotrophic Factor (BDNF), Neurotrophin-3 and Neurotrophin-4/5. NGF R is a 75 kDa protein that is expressed in neuronal axons, Schwann’s cells and perineural cells of peripheral nerves (1). Neural crest stem cells have been isolated based on their surface expression of NGF R (2, 3). In addition, neuroepithelial-derived NGF R positive cells have also been demonstrated to be able to differentiate into neurons, smooth muscle and Schwann cells in culture (4). NGF R has been used as a marker to identify mesenchymal precursors as well as hepatic stellate cells (5, 6).

1. Barker, P.A. et al. (1992) Mol. Cell Biochem. 110:1.
2. Stemple, D.L. et al. (1992) Cell 71:973.
3. Morrison, S.J. et al. (1999) Cell 96:737.
4. Mujtaba, T. et al. (1998) Dev. Biol. 200:1.
5. Campagnolo, L. et al. (2001) Biol. Reprod. 64:464.
6. Cassiman, D. et al. (2001) Hepatology 33:148.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NGFR/TNFRSF16/p75NTR Antibody (MAB367)(6)

Publications using MAB367

• J Wang, A Wuethrich, AA Sina, RE Lane, LL Lin, Y Wang, J Cebon, A Behren, M Trau Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma Sci Adv, 2020;6(9):eaax3223. 2020 [PMID: 32133394] (Flow Cytometry, Human)
• Rump L, Mattey D, Kehoe O, Middleton J An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium. Cytokine, 0;97(0):133-140. 0 [PMID: 28648867] (IHC-Fr, Human)
• Decreased demand for olfactory periglomerular cells impacts on neural precursor cell viability in the rostral migratory stream Sci Rep, 2016;6(0):32203. 2016 [PMID: 27573347] (IHC, Mouse)
• Xi R, Zhang X, Chen X, Pan S, Hui B, Zhang L, Fu S, Li X, Zhang X, Gong T, Guo J, Che S Human papillomavirus 16 infection predicts poor outcome in patients with esophageal squamous cell carcinoma. Onco Targets Ther, 2015;8(0):573-81. 2015 [PMID: 25784817] (IHC-P, Human)
• Berzi A, Ayata CK, Cavalcante P, Falcone C, Candiago E, Motta T, Bernasconi P, Hohlfeld R, Mantegazza R, Meinl E, Farina C BDNF and its receptors in human myasthenic thymus: implications for cell fate in thymic pathology. J. Neuroimmunol., 2008;197(2):128-39. 2008 [PMID: 18555538] (ICC, Human)
• Garderet L, Mazurier C, Chapel A, Ernou I, Boutin L, Holy X, Gorin NC, Lopez M, Doucet C, Lataillade JJ Mesenchymal stem cell abnormalities in patients with multiple myeloma. Leuk. Lymphoma, 2007;48(10):2032-41. 2007 [PMID: 17917971] (Flow Cytometry, Human)

Review for NGFR/TNFRSF16/p75NTR Antibody (MAB367) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Christian Schuerch IHC-Fr Human 02/29/2020

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: human tonsil
• Species: Human
• Lot: N/A

Comments

• Comments: pH 9 epitope retrieval

Bioinformatics

• Gene Symbol: NGFR
• Uniprot:
  • P08138()