NGFR/TNFRSF16/p75NTR Antibody (74902) [Unconjugated]

Images

 
NGF R/TNFRSF16 was detected in immersion fixed canine mesenchymal stem cells using Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
NGF R/TNFRSF16 was detected in immersion fixed human mesenchymal stem cells using Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
NGF R/TNFRSF16 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 25 µg/mL Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) overnight at 4 °C. Tissue ...read more
Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) followed by ...read more
SH‑SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed ...read more

Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, ICC/IF
Clone
74902
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

NGFR/TNFRSF16/p75NTR Antibody (74902) [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NGF R
Lys29-Asn250
Accession # P08138
Specificity
Detects human NGF R in direct ELISAs and Western blots. In direct ELISAs, no crossreactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD40, rhBAFF R, rhCD30, rhDR3, rhDR6, rhEDAR, rhFas, rhHVEM, rhGITR, rhLTR B, recominant mouse (rm) NGF R, rhOPG, rmOX40, rhRANK, rhTAJ, rhTNF RI or rhTNF RII is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NGFR
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB367 in the following applications:

Publications
Read Publications using
MAB367 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NGFR/TNFRSF16/p75NTR Antibody (74902) [Unconjugated]

  • CD271 antigen
  • CD271
  • Gp80-LNGFR
  • member 16
  • nerve growth factor receptor
  • NGF R
  • NGF receptor
  • NGFR
  • p75 ICD
  • p75 NTR
  • TNFRSF16
  • TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16)
  • tumor necrosis factor receptor superfamily member 16

Background

NGF R is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family (1) and has been designated TNFRSF16. This receptor is also known as p75 NTR (neurotrophin receptor) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including Brain-Derived Neurotrophic Factor (BDNF), Neurotrophin-3 and Neurotrophin-4/5. NGF R is a 75 kDa protein that is expressed in neuronal axons, Schwann’s cells and perineural cells of peripheral nerves (1). Neural crest stem cells have been isolated based on their surface expression of NGF R (2, 3). In addition, neuroepithelial-derived NGF R positive cells have also been demonstrated to be able to differentiate into neurons, smooth muscle and Schwann cells in culture (4). NGF R has been used as a marker to identify mesenchymal precursors as well as hepatic stellate cells (5, 6).
  1. Barker, P.A. et al. (1992) Mol. Cell Biochem. 110:1.
  2. Stemple, D.L. et al. (1992) Cell 71:973.
  3. Morrison, S.J. et al. (1999) Cell 96:737.
  4. Mujtaba, T. et al. (1998) Dev. Biol. 200:1.
  5. Campagnolo, L. et al. (2001) Biol. Reprod. 64:464.
  6. Cassiman, D. et al. (2001) Hepatology 33:148.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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    Publications for NGFR/TNFRSF16/p75NTR Antibody (MAB367)(6)

    We have publications tested in 2 confirmed species: Human, Mouse.

    We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P.


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    Flow Cytometry
    (2)
    ICC
    (1)
    IHC
    (1)
    IHC-Fr
    (1)
    IHC-P
    (1)
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    Filter By Species
    Human
    (5)
    Mouse
    (1)
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    Showing Publications 1 - 6 of 6.
    Publications using MAB367 Applications Species
    J Wang, A Wuethrich, AA Sina, RE Lane, LL Lin, Y Wang, J Cebon, A Behren, M Trau Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma Sci Adv, 2020;6(9):eaax3223. 2020 [PMID: 32133394] (Flow Cytometry, Human) Flow Cytometry Human
    Rump L, Mattey D, Kehoe O, Middleton J An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium. Cytokine, 0;97(0):133-140. 0 [PMID: 28648867] (IHC-Fr, Human) IHC-Fr Human
    Decreased demand for olfactory periglomerular cells impacts on neural precursor cell viability in the rostral migratory stream Sci Rep, 2016;6(0):32203. 2016 [PMID: 27573347] (IHC, Mouse) IHC Mouse
    Xi R, Zhang X, Chen X, Pan S, Hui B, Zhang L, Fu S, Li X, Zhang X, Gong T, Guo J, Che S Human papillomavirus 16 infection predicts poor outcome in patients with esophageal squamous cell carcinoma. Onco Targets Ther, 2015;8(0):573-81. 2015 [PMID: 25784817] (IHC-P, Human) IHC-P Human
    Berzi A, Ayata CK, Cavalcante P, Falcone C, Candiago E, Motta T, Bernasconi P, Hohlfeld R, Mantegazza R, Meinl E, Farina C BDNF and its receptors in human myasthenic thymus: implications for cell fate in thymic pathology. J. Neuroimmunol., 2008;197(2):128-39. 2008 [PMID: 18555538] (ICC, Human) ICC Human
    Garderet L, Mazurier C, Chapel A, Ernou I, Boutin L, Holy X, Gorin NC, Lopez M, Doucet C, Lataillade JJ Mesenchymal stem cell abnormalities in patients with multiple myeloma. Leuk. Lymphoma, 2007;48(10):2032-41. 2007 [PMID: 17917971] (Flow Cytometry, Human) Flow Cytometry Human

    Review for NGFR/TNFRSF16/p75NTR Antibody (MAB367) (1) 41

    Average Rating: 4
    (Based on 1 review)
    We have 1 review tested in 1 species: Human.

    Reviews using MAB367:
    Filter by Applications
    IHC-Fr
    (1)
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    Filter by Species
    Human
    (1)
    All Species
    Images Ratings Applications Species Date Details
    Immunohistochemistry-Frozen NGFR/TNFRSF16/p75NTR MAB367
    Enlarge
    4
    reviewed by:
    Christian Schuerch
    IHC-Fr Human 02/29/2020
    View

    Summary

    ApplicationImmunohistochemistry-Frozen
    Sample Testedhuman tonsil
    SpeciesHuman
    LotN/A

    Comments

    CommentspH 9 epitope retrieval

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    Secondary Antibodies

     

    Isotype Controls

    Other Available Formats

    Alexa Fluor 350 FAB367U
    Alexa Fluor 405 FAB367V
    Alexa Fluor 488 FAB367G
    Alexa Fluor 532 FAB367X
    Alexa Fluor 594 FAB367T
    Alexa Fluor 647 FAB367R
    Alexa Fluor 700 FAB367N
    Alexa Fluor 750 FAB367S
    Allophycocyanin FAB367A
    DyLight 350 FAB367D
    DyLight 405 FAB367E
    DyLight 488 FAB367K
    DyLight 550 FAB367L
    DyLight 594 FAB367M
    DyLight 650 FAB367W
    DyLight 680 FAB367Y
    DyLight 755 FAB367Z
    FITC FAB367F
    HRP FAB367H
    Janelia Fluor 549 FAB367I
    Janelia Fluor 646 FAB367J
    PerCP FAB367C

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    Recent Reviews

    4
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    Christian Schuerch
    02/29/2020
    Application: IHC-Fr
    Species: Human

    Bioinformatics

    Gene Symbol NGFR
    Uniprot