NGFI-B alpha/Nur77/NR4A1 Antibody (H1648) [Unconjugated]

• Reactivity: Hu
• Applications: WB, IP, ELISA
• Clone: H1648
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Suitable Sample Type: 23015

NGFI-B alpha/Nur77/NR4A1 Antibody (H1648) [Unconjugated] Summary

• Immunogen: Recombinant human NGFI-B alpha/Nur77/NR4A1
  aa 2-249
• Specificity: This antibody specifically recognizes human NGFI-B alpha but does not recognize NGFI-B beta and gamma. Not yet tested in other species.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR4A1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
  • Immunoprecipitation
  • Direct ELISA

Packaging, Storage & Formulations

• Storage: This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
• Buffer: A liquid formulation in physiologic saline with 0.1% NaN₃.
• Preservative: 0.1% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NGFI-B alpha/Nur77/NR4A1 Antibody (H1648) [Unconjugated]

• Early response protein NAK1
• GFRP1ST-59
• growth factor-inducible nuclear protein N10
• HMR
• HMRNP10
• hormone receptor
• MGC9485
• N10
• NAK1
• NAK-1
• NGFIB alpha
• NGFI-B alpha
• NGFIB
• NR4A1
• Nuclear hormone receptor NUR/77
• nuclear receptor subfamily 4 group A member 1
• nuclear receptor subfamily 4, group A, member 1
• Nur77
• Orphan nuclear receptor HMR
• Orphan nuclear receptor TR3
• steroid receptor TR3
• Testicular receptor 3
• TR3 orphan receptor
• TR3

Background

Nerve growth factor inducible factor I-B alpha (NGFI-B alpha, Nur77, TR3, HMR; NR4A1) is a member of the Orphan Nuclear Receptor family. NGFI-B alpha is expressed in the brain, adrenals, thyroid, liver, testis, ovary, thymus, muscle, lung and prostate. Its expression is induced in response to various stress stimuli and growth factors. NGFI-B alpha is involved in mediating apoptotic signaling in thymocytes and tumor cells, as well as having a role in signaling in the hypothalamic-pituitary axis. RXR has been shown to be a partner for NGFI-B alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NGFI-B alpha/Nur77/NR4A1 Antibody (PP-H1648-00)(2)

Publications using PP-H1648-00

• Liu J, Zhou W, Li SS, Sun Z, Lin B, Lang YY, He JY, Cao X, Yan T, Wang L, Lu J, Han YH, Cao Y, Zhang XK, Zeng JZ Modulation of orphan nuclear receptor Nur77-mediated apoptotic pathway by acetylshikonin and analogues. Cancer Res., 2008;68(21):8871-80. 2008 [PMID: 18974131] (ICC, Human)
• Luciano F, Krajewska M, Ortiz-Rubio P, Krajewski S, Zhai D, Faustin B, Bruey JM, Bailly-Maitre B, Lichtenstein A, Kolluri SK, Satterthwait AC, Zhang XK, Reed JC Nur77 converts phenotype of Bcl-B, an antiapoptotic protein expressed in plasma cells and myeloma. Blood, 2007;109(9):3849-55. 2007 [PMID: 17227826] (Immunoprecipitation, Human)

Bioinformatics

• Gene Symbol: NR4A1