NFkB1/NFkB p105 Antibody

Images

 
Western Blot: NFkB1/NFkB p105 Antibody [NBP1-77395] - WB analysis of NFkB in HepG2 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: NFkB1/NFkB p105 Antibody [NBP1-77395] - Antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: NFkB1/NFkB p105 Antibody [NBP1-77395] - IHC analysis of NFKB in mouse kidney using DAB with hematoxylin counterstain.
Simple Western: NFkB1/NFkB p105 Antibody [NBP1-77395] - Simple Western lane view shows a specific band for NFkB p105 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.18 mg/ml

NFkB1/NFkB p105 Antibody Summary

Immunogen
A genomic peptide made to an N-terminal region of the human NFkB p105/p50 protein (within residues 400-590). [Swiss-Prot P19838]
Localization
Nucleus and cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFKB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:20
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Paraffin 1:250
Application Notes
This NFKB1 antibody is useful for IHC-P, ICC/IF and Western blot where a band is seen approx. 110 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
MCF-7 Lysate (NB800-PC5)
Publications
Read Publications using
NBP1-77395 in the following applications:

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.18 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for NFkB1/NFkB p105 Antibody

  • DNA-binding factor KBF1
  • EBP-1
  • NF-kappa-B
  • NFkB1
  • NF-kB1
  • NFKB-p105
  • Nuclear factor NF-kappa-B p105 subunit
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
  • p105

Background

Nuclear factor kappa beta (NFKB) is a transcription factor that is highly influenced by stress, free-radicals, UV light and hypoxia. Unsurprisingly, NFKB is essential for many human cancers, as it regulates the expression of many downstream proteins that regulate or modify cellular growth and development. NFKB effects on angiogenic pathways and reactive mechanisms to stress and damage are well established throughout literature. Atherosclerosis is partly attributed to the mis-regulation of NFKB in hypoxia. Arthritis is related to inflammation proteins regulated through NFKB. It also has been shown to be important in synapses in brain, leading to memory and cognitive ability or impairment. In all, NFKB is one of the fastest responding transcription factors in humans, and is likely to mediate many responsive events in diseases and injury recovery.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NFkB1/NFkB p105 Antibody (NBP1-77395)(3)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NFkB1/NFkB p105 Antibody (NBP1-77395). (Showing 1 - 1 of 1 FAQs).

  1. I need a NFKappa B antibody p105 that recognizes both p105 and p50. In the past I used the Novus Biological antibody NBP1-77395 but I am not sure if it recognizes p50 as well?
    • We have not tested product NBP1-77395 specifically for the p50 subunit. The immunogen is made to aa 400-590 of the human NFkB p105/p50 protein. The p150 subunit encompasses aa 1-968 and the p50 subunit encompasses aa 1-433. Since the immunogen sequence does not include the entire p50 subunit, I do not think it will detect it. We have several products that should detect both subunits, which you can view here: available products.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-77395AF405
Alexa Fluor 488 Labeled NBP1-77395AF488
Alexa Fluor 647 Labeled NBP1-77395AF647
Alexa Fluor 700 Labeled NBP1-77395AF700
Biotin Labeled NBP1-77395B
DyLight 405 Labeled NBP1-77395V
DyLight 488 Labeled NBP1-77395G
DyLight 550 Labeled NBP1-77395R
DyLight 650 Labeled NBP1-77395C
FITC Labeled NBP1-77395F
HRP Labeled NBP1-77395H

Additional NFkB1/NFkB p105 Products

NFkB1/NFkB p105 NBP1-77395

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Bioinformatics

Gene Symbol NFKB1
Entrez
Uniprot