NFkB p100/p52 Antibody (S04-4D6)

Immunocytochemistry/Immunofluorescence: NFkB p100/p52 Antibody (S04-4D6) [NBP3-14959] - Immunofluorescence of NFkB p100/p52 (green) in Hela cells using NFkB p100/p52 Rabbit mAb at dilution 1/200, and DAPI (blue)

Western Blot: NFkB p100/p52 Antibody (S04-4D6) [NBP3-14959] - Western blot detection of C6, 3T3, Hela cell lysates using NBP3-14959. Predicted band size: 97kDa. Observed band size: 100kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: S04-4D6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

NFkB p100/p52 Antibody (S04-4D6) Summary

• Description: Novus Biologicals Rabbit NFkB p100/p52 Antibody (S04-4D6) (NBP3-14959) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of human NFkB p100/p52 (Uniprot#: Q00653)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NFKB2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:1000-1:2000
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4), 150mM sodium chloride, 0.05% BSA, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NFkB p100/p52 Antibody (S04-4D6)

• DNA-binding factor KBF2
• H2TF1
• Lymphocyte translocation chromosome 10 protein
• Lyt10
• LYT10LYT-10
• nuclear factor NF-kappa-B p100 subunit
• nuclear factor of kappa light chain gene enhancer in B-cells 2
• nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
• Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2
• Oncogene Lyt-10
• p52

Background

The NFkappaB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The DNA binding subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFkappaB. It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFkappaB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFKB2