NFIL3 Antibody (1218A) [Unconjugated]

Mouse splenocytes treated with 100 ng/mL Recombinant Mouse IL‑2 (Catalog # 402-ML) overnight were stained with Rat Anti-Mouse NKp46/NCR1 APC‑conjugated Monoclonal Antibody (Catalog # FAB22252A) and either (A) Rabbit Anti-Mouse NFIL3/E4BP4 Monoclonal Antibody (Catalog # MAB8888) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

• Reactivity: Mu
• Applications: Flow
• Clone: 1218A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

NFIL3 Antibody (1218A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant mouse NFIL3/E4BP4
  Lys140-Arg462
  Accession # O08750
• Specificity: Detects NFIL3/E4BP4 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: Nfil3
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NFIL3 Antibody (1218A) [Unconjugated]

• E4BP4
• E4BP4IL3BP1
• IL3BP1
• Interleukin-3 promoter transcriptional activator
• Interleukin-3-binding protein 1
• NFIL3
• NF-IL3A
• NFIL3AE4 promoter-binding protein 4
• nuclear factor interleukin 3 regulated protein
• nuclear factor interleukin-3-regulated protein
• nuclear factor, interleukin 3 regulated
• Transcriptional activator NF-IL3A

Background

NFIL3 (Nuclear Factor, Interleukin 3 Regulated), also called E4BP4 (adenovirus E4 promoter binding protein 4), is an approximately 58 kDa transcription factor of the bZIP (basic leucine zipper) family. It is a transcriptional regulator expressed in T and pro-B lymphocytes and cardiomyocytes, binds IL-3 and promoters as a homodimer, and enhances cell survival. NFIL3 has also been shown to be involved in the development of Innate Lymphoid Cells (ILCs; 1,2). The 462 amino acid (aa) human NFIL3 contains a basic DNA binding domain (aa 73-95), a leucine zipper (aa 96-124), and a transcriptional repression domain (aa 299-363) that is potentially regulated by phosphorylation at Ser301 and Ser353. Within the region used as an immunogen, human NFIL3 shares 83% aa sequence identity with mouse and rat NFIL3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NFIL3 Antibody (MAB8888)(1)

Publication using MAB8888

• CS Umeshappa, P Solé, J Yamanouchi, S Mohapatra, BGJ Surewaard, J Garnica, S Singha, D Mondal, E Cortés-Vic, C D'Mello, A Mason, P Kubes, P Serra, Y Yang, P Santamaria Re-programming mouse liver-resident invariant natural killer T cells for suppressing hepatic and diabetogenic autoimmunity Nature Communications, 2022-06-07;13(1):3279. 2022-06-07 [PMID: 35672409] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: Nfil3
• Uniprot:
  • O08750()