NFATC2/NFAT1 Antibody (639402)

Western blot shows lysates of BJAB human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Human NFATC2 Monoclonal Antibody (Catalog # MAB6499) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NFATC2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 639402
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NFATC2/NFAT1 Antibody (639402) Summary

• Immunogen: E. coli-derived recombinant human NFATC-2
  His575-Pro679
  Accession # Q13469
• Specificity: Detects human NFATC2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human NFATC1 or C3 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NFATC2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NFATC2/NFAT1 Antibody (639402)

• NFAT pre-existing subunit
• NFAT transcription complex, preexisting component
• NFAT1
• NFAT1nuclear factor of activated T-cells, cytoplasmic 2
• NFATC2
• NF-ATc2
• NFATP
• NF-ATp
• NFATPnuclear factor of activated T-cells, preexisting component
• nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
• preexisting nuclear factor of activated T-cells 2
• T cell transcription factor NFAT1
• T-cell transcription factor NFAT1

Background

NFATC2 (Nuclear factor of activated T cells C2; also NFAT1 or NFAT2P) is a 135 kDa member of the NFAT family of transcription factors. NFATC2 is found in T cells and mast cells where it regulates cytokine transcription, Th2 cell differentiation, and cell cycle entry. The transactivation function of NFATC2 is regulated by phosphorylation at Ser53, Ser56, Thr116, and Ser170. Human NFATC2 is 925 amino acids (aa) in length. It contains a calcineurin-binding site (aa 111-116), a transactivation domain (aa 119-199), two NLS (aa 251-253 and 664-666), an RHD that binds DNA (aa 392-574), and one NES (aa 904-913). An alternate splice form has a substitution of the C-terminal 18 amino acids. Human NFATC2 shares 96% aa identity with mouse and rat NFATC2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NFATC2/NFAT1 Antibody (MAB6499)(1)

Publication using MAB6499

• Yanjiao Huang, Qiang Li, Zunyong Feng, Lanrong Zheng STIM1 controls calcineurin/Akt/mTOR/NFATC2‑mediated osteoclastogenesis induced by RANKL/M‑CSF Experimental and Therapeutic Medicine 2020-05-19 [PMID: 32742319]

Bioinformatics

• Gene Symbol: NFATC2
• Uniprot:
  • Q13469()