NFATC2/NFAT1 Antibody (639402) [Alexa Fluor® 647]

• Applications: WB
• Clone: 639402
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

NFATC2/NFAT1 Antibody (639402) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human NFATC-2
  His575-Pro679
  Accession # Q13469
• Specificity: Detects human NFATC2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human NFATC1 or C3 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

NFATC2 (Nuclear factor of activated T cells C2; also NFAT1 or NFAT2P) is a 135 kDa member of the NFAT family of transcription factors. NFATC2 is found in T cells and mast cells where it regulates cytokine transcription, Th2 cell differentiation, and cell cycle entry. The transactivation function of NFATC2 is regulated by phosphorylation at Ser53, Ser56, Thr116, and Ser170. Human NFATC2 is 925 amino acids (aa) in length. It contains a calcineurin-binding site (aa 111-116), a transactivation domain (aa 119-199), two NLS (aa 251-253 and 664-666), an RHD that binds DNA (aa 392-574), and one NES (aa 904-913). An alternate splice form has a substitution of the C-terminal 18 amino acids. Human NFATC2 shares 96% aa identity with mouse and rat NFATC2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.