| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-504-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82583-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6499-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82582-25ul | |||
![Immunocytochemistry/Immunofluorescence: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Analysis of NFATc2 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were left untreated (left panel) or treated with 1uM staurosporine (right panel) for 3 hours and probed with a NFATc2 monoclonal antibody, at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-25A10-D6-D2-Immunocytochemistry-Immunofluorescence-NB300-504-img0010.jpg "Immunocytochemistry/Immunofluorescence: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Analysis of NFATc2 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were left untreated (left panel) or treated with 1uM staurosporine (right panel) for 3 hours and probed with a NFATc2 monoclonal antibody, at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.")
![Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Both normal and cancer biopsies of deparaffinized Human spleen tissue tissues.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-25A10-D6-D2-Immunohistochemistry-Paraffin-NB300-504-img0012.jpg)
![Chromatin Immunoprecipitation: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - IP performed using Matrix ChIP assay with 1.0ul/100ul well of NFATc2 mab. Chromatin aliquots from ~1x10^5 cells used per ChIP pull-down. Quantitative PCR data done x4 using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify exon-1 or exon-28 of LAMC1. PCR calibration curves generated per primer pair from a series of sheared total genomic DNA. ChIP presented relative to the total amount of input chromatin. Results represent mean +/- SEM for 3 experiments. Representation of rat LAMC1 locus shown above data where boxes represent exons (black = translated regions, white = untranslated regions); zigzag line represents an intron, & straight line represents upstream sequence. Regions amplified by LAMC1 primers are represented by black bars. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-25A10-D6-D2-Chromatin-Immunoprecipitation-NB300-504-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Analysis of NFATc2 using NFATc2 Monoclonal Antibody (25A10.D6.D2) shows staining in Hela Cells. NFATc2 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc2 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-25A10-D6-D2-Immunocytochemistry-Immunofluorescence-NB300-504-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Analysis of NFATc2 using NFATc2 Monoclonal Antibody (25A10.D6.D2) shows staining in MCF-7 Cells. NFATc2 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc2 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-25A10-D6-D2-Immunocytochemistry-Immunofluorescence-NB300-504-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Analysis of NFATc2 using NFATc2 Monoclonal Antibody (25A10.D6.D2) shows staining in U251 Cells. NFATc2 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc2 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-25A10-D6-D2-Immunocytochemistry-Immunofluorescence-NB300-504-img0008.jpg)
![Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Cancer biopsies of deparaffinized Human colon carcinoma tissues.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-25A10-D6-D2-Immunohistochemistry-Paraffin-NB300-504-img0004.jpg)
![Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-25A10-D6-D2-Immunohistochemistry-Paraffin-NB300-504-img0011.jpg)
![Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-82583] - Analysis in human lymph node and skeletal muscle tissues. Corresponding NFATC2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-82583-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-82583] - Analysis in human lymph node and skeletal muscle tissues. Corresponding NFATC2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: NFATC2/NFAT1 Antibody [NBP1-82583] - Analysis in human cell line CACO-2.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-Western-Blot-NBP1-82583-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: NFATC2/NFAT1 Antibody [NBP1-82583] - Staining of human cell line U-251 MG shows localization to nucleoplasm and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82583-img0010.jpg)
![Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-82583] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-82583-img0014.jpg)
![Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-82583] - Staining of human lymph node shows positivity in lymphoid cells.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-82583-img0012.jpg)
![Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-82583] - Staining of human duodenum shows positivity in lymphoid cells.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-82583-img0013.jpg)
![Western Blot: NFATC2/NFAT1 Antibody [NBP1-51970] - NBP1-51970 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-Western-Blot-NBP1-51970-img0004.jpg "Western Blot: NFATC2/NFAT1 Antibody [NBP1-51970] - NBP1-51970 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-51970] - NBP1-51970 (3.8ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-51970-img0003.jpg)
![Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-82582] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-82582-img0007.jpg "Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-82582] - Staining of human lymph node shows high expression.")
![Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-82582] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-82582-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-82582] - Staining in human lymph node and cerebral cortex tissues using anti-NFATC2 antibody. Corresponding NFATC2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-82582-img0008.jpg)
![Western Blot: NFAT1 Overexpression Lysate (Adult Normal) [NBP2-05154] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NFAT1.](http://images.novusbio.com/fullsize/NFAT1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05154-img0001.jpg "Western Blot: NFAT1 Overexpression Lysate (Adult Normal) [NBP2-05154] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NFAT1.")
![Western Blot: NFAT1 Overexpression Lysate (Adult Normal) [NBP2-10355] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NFAT1.](http://images.novusbio.com/fullsize/NFAT1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10355-img0001.jpg "Western Blot: NFAT1 Overexpression Lysate (Adult Normal) [NBP2-10355] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NFAT1.")
![Sandwich ELISA: NFATC2/NFAT1 Antibody (2A4) [H00004773-M01] - Detection limit for recombinant GST tagged NFATC2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-2A4-Sandwich-ELISA-H00004773-M01-img0002.jpg "Sandwich ELISA: NFATC2/NFAT1 Antibody (2A4) [H00004773-M01] - Detection limit for recombinant GST tagged NFATC2 is approximately 0.1ng/ml as a capture antibody.")
![ELISA: Human NFATC2/NFAT1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39197] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39197_human-nfatc2-nfat1-ready-to-use-elisa-kit-colorimetric-211020241530299.png "ELISA: Human NFATC2/NFAT1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39197] - Standard Curve Reference")
![ELISA: Human NFATC2/NFAT1 ELISA Kit (Colorimetric) [NBP3-39196] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39196_human-nfatc2-nfat1-elisa-kit-colorimetric-2110202415384084.png "ELISA: Human NFATC2/NFAT1 ELISA Kit (Colorimetric) [NBP3-39196] - Standard Curve Reference")