NF-L Antibody

Western Blot: NF-L Antibody [H00004747-D01P] - Analysis of NEFL expression in Hela S3 NE.

Western Blot: NF-L Antibody [H00004747-D01P] - Analysis of NEFL expression in transfected 293T cell line by NEFL polyclonal antibody.Lane 1: NEFL transfected lysate(61.50 KDa).Lane 2: Non-transfected lysate.

Proximity Ligation Assay: NF-L Antibody [H00004747-D01P] - Analysis of protein-protein interactions between NEFL and APP. HeLa cells were stained with anti-NEFL rabbit purified polyclonal 1:1200 and anti-APP mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Discontinued

NF-L Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: NEFL (NP_006149.2, 1 a.a. - 543 a.a.) full-length human protein. MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD
• Marker: Neuronal Marker
• Specificity: NEFL - neurofilament, light polypeptide 68kDa,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEFL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on WB and also on transfected lysate in WB. GST tag alone is used as a negative control. ELISA usage reported in scientific literature (PMID: 23287695).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NF-L Antibody

• 68 kDa neurofilament protein
• CMT2E
• NEFL
• neurofilament protein, light chain
• neurofilament subunit NF-L
• Neurofilament triplet L protein
• neurofilament, light polypeptide
• neurofilament-light
• NF68
• NF68FLJ53642
• NFL
• NF-L
• NFLlight polypeptide 68kDa

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NF-L Antibody (H00004747-D01P)(2)

Publications using H00004747-D01P

• Rana OR, Schroder JW, Baukloh JK et al. Neurofilament light chain as an early and sensitive predictor of long-term neurological outcome in patients after cardiac arrest Int J Cardiol 2012-12-31 [PMID: 23287695] (ELISA, Human)
• Madeddu R, Farace C, Tolu P et al. Cytoskeletal proteins in the cerebrospinal fluid as biomarker of multiple sclerosis. Neurol Sci. 2012-02-24 [PMID: 22362332] (Human)

Bioinformatics

• Gene Symbol: NEFL
• Entrez:
  • 4747(Human)
• Uniprot:
  • NP_006149.2(Human)