NF-L Antibody (NFL/736) - IHC-Prediluted

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Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) - IHC-Prediluted [NBP2-48294] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).

Product Details

Summary
Reactivity Hu, Rt, Po, Ch, PmSpecies Glossary
Applications IHC, IHC-P
Clone
NFL/736
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
IHC-Prediluted

Order Details

NF-L Antibody (NFL/736) - IHC-Prediluted Summary

Immunogen
Recombinant human NEFL protein
Localization
Cytoplasmic
Marker
Neuronal Marker
Specificity
This MAb reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NEFL
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Hu-chromosome location: 8p21.2 Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Alternate Names for NF-L Antibody (NFL/736) - IHC-Prediluted

  • 68 kDa neurofilament protein
  • CMT2E
  • NEFL
  • neurofilament protein, light chain
  • neurofilament subunit NF-L
  • Neurofilament triplet L protein
  • neurofilament, light polypeptide
  • neurofilament-light
  • NF68
  • NF68FLJ53642
  • NFL
  • NF-L
  • NFLlight polypeptide 68kDa

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47970AF350
Alexa Fluor 405 NBP2-47970AF405
Alexa Fluor 488 NBP2-47970AF488
Alexa Fluor 532 NBP2-47970AF532
Alexa Fluor 594 NBP2-47970AF594
Alexa Fluor 647 NBP2-47970AF647
Alexa Fluor 700 NBP2-47970AF700
Alexa Fluor 750 NBP2-47970AF750
Biotin NBP2-47970B
DyLight 350 NBP2-47970UV
DyLight 405 NBP2-47970V
DyLight 488 NBP2-47970G
DyLight 550 NBP2-47970R
DyLight 594 NBP2-47970DL594
DyLight 650 NBP2-47970C
DyLight 680 NBP2-47970FR
DyLight 755 NBP2-47970IR
FITC NBP2-47970F
HRP NBP2-47970H
Janelia Fluor 549 NBP2-47970JF549
Janelia Fluor 646 NBP2-47970JF646

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Bioinformatics

Gene Symbol NEFL