Reactivity | Hu, Rt, Po, Ch, PmSpecies Glossary |
Applications | IHC, IHC-P |
Clone | NFL/736 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Format | IHC-Prediluted |
Immunogen | Recombinant human NEFL protein |
Localization | Cytoplasmic |
Marker | Neuronal Marker |
Specificity | This MAb reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. |
Isotype | IgG1 |
Clonality | Monoclonal |
Host | Mouse |
Gene | NEFL |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Hu-chromosome location: 8p21.2 Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application. |
Theoretical MW | 68 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at 4C. |
Buffer | 10 mM PBS with 0.05% BSA |
Preservative | 0.05% Sodium Azide |
Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Alexa Fluor 350 | NBP2-47970AF350 | |
Alexa Fluor 405 | NBP2-47970AF405 | |
Alexa Fluor 488 | NBP2-47970AF488 | |
Alexa Fluor 532 | NBP2-47970AF532 | |
Alexa Fluor 594 | NBP2-47970AF594 | |
Alexa Fluor 647 | NBP2-47970AF647 | |
Alexa Fluor 700 | NBP2-47970AF700 | |
Alexa Fluor 750 | NBP2-47970AF750 | |
Biotin | NBP2-47970B | |
DyLight 350 | NBP2-47970UV | |
DyLight 405 | NBP2-47970V | |
DyLight 488 | NBP2-47970G | |
DyLight 550 | NBP2-47970R | |
DyLight 594 | NBP2-47970DL594 | |
DyLight 650 | NBP2-47970C | |
DyLight 680 | NBP2-47970FR | |
DyLight 755 | NBP2-47970IR | |
FITC | NBP2-47970F | |
HRP | NBP2-47970H | |
Janelia Fluor 549 | NBP2-47970JF549 | |
Janelia Fluor 646 | NBP2-47970JF646 |
Diseases for NF-L Antibody (NBP2-48294)Discover more about diseases related to NF-L Antibody (NBP2-48294).
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Gene Symbol | NEFL |