NF-H ELISA Kit (Colorimetric)

ELISA: NF-H ELISA Kit [HRP] [KA1479] - Standard curve

Product Discontinued

NF-H ELISA Kit (Colorimetric) Summary

• Description: Quality control test: Standard curve. Phosphorylated NEFH (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of phosphorylated NEFH.
• Sensitivity: 24 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 16.50%
• Intra-Assay: CV% < 3.70%
• Sample Volume: 100 uL (diluted)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NEFH

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification
• Application Notes: Useful for quantification.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NF-H ELISA Kit (Colorimetric)

• 200 kDa neurofilament protein
• KIAA0845
• NEFH
• Neurofilament Heavy (200kDa)
• neurofilament heavy polypeptide
• Neurofilament triplet H protein
• neurofilament, heavy polypeptide 200kDa
• neurofilament, heavy polypeptide
• NF200
• NFH
• NF-H

Background

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220kDa, with some size variation in across species.

Antibodies to NF-H may be used to identify NF-H in neurons and to study neuronal processes in tissue sections and/or culture. NF-H antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for NF-H ELISA Kit (KA1479)(2)

Publications using KA1479

• Hakansson I, Tisell A, Cassel P et al. Neurofilament levels, disease activity and brain volume during follow-up in multiple sclerosis. J Neuroinflammation 2018-07-18 [PMID: 30021640]
• Hakansson I, Tisell A, Cassel P et al. Neurofilament light chain in cerebrospinal fluid and prediction of disease activity in clinically isolated syndrome and relapsing-remitting multiple sclerosis. Eur J Neurol 2017-03-06 [PMID: 28261960]

FAQs for NF-H ELISA Kit (KA1479). (Showing 1 - 2 of 2 FAQs).

1. Is your NF-H ELISA predicted to work on samples from horses?
   • The ELISA kit KA1479 has only been tested for detection of the human Phosphorylated NEFH protein. It has not yet been tested for cross reactivity to other species at this time.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NEFH