NF-H Antibody (NF-200)

Product Discontinued

NF-H Antibody (NF-200) Summary

• Immunogen: C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.
• Marker: Neuronal Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEFH
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Frozen 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1:100-1:2000

Reactivity Notes

Human, Mouse, Rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1.2% Sodium Acetate and 0.2 mg BSA
• Preservative: 0.01 mg Sodium Azide
• Concentration: LYOPH
• Purity: Protein A or G purified
• Reconstitution Instructions: Reconstitute with deionized water.

Alternate Names for NF-H Antibody (NF-200)

• 200 kDa neurofilament protein
• KIAA0845
• NEFH
• Neurofilament Heavy (200kDa)
• neurofilament heavy polypeptide
• Neurofilament triplet H protein
• neurofilament, heavy polypeptide 200kDa
• neurofilament, heavy polypeptide
• NF200
• NFH
• NF-H

Background

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for NF-H Antibody (NBP1-42578). (Showing 1 - 1 of 1 FAQ).

1. The suggested dilution titer for WB with NBP1-42578? The product is lyophilized, and there is no suggestion for WB. The customer wonders how much diH2O shall be added for reconstitution?
   • To reconstitute to the Ab the customer should add distilled H20 for the desired concentration. Generally, a concentration of 1.0 mg/ml is appropriate. For Western blot we recommend a starting dilution of 1:100 - 1:2000. The optimal dilution will be dependent on how much protein is in the sample.

Bioinformatics

• Gene Symbol: NEFH
• Entrez:
  • 4744(Human)
  • 380684(Mouse)
• Uniprot:
  • P12036()