Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard curve. Elastase Ab ELISA Kit is an indirect solid phase ELISA for the quantitative measurement of IgG class autoantibodies against Elastase in human serum or plasma. |
| Standard Curve Range |
6.3 to 100 U/mL |
| Sensitivity |
0.5 U/mL |
| Assay Type |
Indirect ELISA |
| Inter-Assay |
CV% < 4.6% |
| Intra-Assay |
CV% < 3.9% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
ELANE |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
Kit Components
|
Components
|
- Calibrators A-F (0, 6.3, 12.5, 25, 50, 100 U/mL), containing serum/buffer matrix (PBS,BSA, detergent, NaN3 0.09%), yellow. Ready to use.
- Control positive (1) and negative (2), containing elastase antibodies in a serum/buffermatrix (PBS, BSA, detergent, NaN3 0.09%), yellow, Ready to use. The concentration isspecified on the certificate of analysis.
- Divisible microplate consisting of 12 modules of 8 wells each. Ready to use.
- Enzyme conjugate, containing anti-human IgG antibodies, HRP labelled; PBS, BSA,detergent, preservative ProClin 300 0.05%, light red. Ready to use.
- Sample buffer P, containing PBS, BSA, detergent, preservative NaN3 0.09%, yellow,concentrate (5x).
- Stop solution, contains acid. Ready to use.
- TMB substrate, containing 3,3’,5,5’-Tetramethylbenzidin, colorless. Ready to use.
- Wash solution, containing Tris, detergent, preservative NaN3 0.09%; 50x concentrated.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric)
Background
The ELANE gene (commonly known as neutrophil elastase) encodes a 267 amino acid long, 28 kDA neutrophil elastase protein that functions in regulation of natural killer cells, monocytes, and graulocytes. ELANE is involved in activation of proMMP8, cell adhesion cell-matrix glycoconjugates, selected targets of C/EBPbeta, degradation of the extracellular matrix and collagen, and amb2 integrin signaling. It interacts with various genes GZMB, LRP1, SERPINF2, SERPING1, and SERPINA1. Elane has been linked to cystic fibrosis, asthma, pneumonia, alzheimer's disease, neutropenia, cutis laxia, leg ulcers, wegener's granulomatosis, bronchitis, adult respiratory distress syndrome, and vascular diseases.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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FAQs for Neutrophil Elastase/ELA2 ELISA Kit (KA1102). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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