Kallikrein 6/Neurosin Antibody

Kallikrein 6/Neurosin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human Kallikrein 6/Neurosin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2008) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Kallikrein 6/Neurosin Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Kallikrein 6
  Glu17-Lys244
  Accession # Q92876
• Specificity: Detects human Kallikrein 6 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Kallikrein 3, rhKallikrein 5, rhKallikrein 11, rhKallikrein 13, rhKallikrein 14, and rhKallikrein 15 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KLK6
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kallikrein 6/Neurosin Antibody

• Bssp
• EC 3.4.21
• EC 3.4.21.-
• hK6
• kallikrein 6 (neurosin, zyme)
• Kallikrein 6
• kallikrein-6
• kallikrein-related peptidase 6
• KLK6
• Klk7
• Neurosin
• Protease M
• protease, serine, 18
• PRSS18
• PRSS9
• PRSS9MGC9355
• Serine protease 18
• Serine protease 9
• SP59
• Zyme

Background

Tissue kallikreins are a family of extracellular serine proteases consisting of 15 members (1, 2). The genes of this family aligned in tandem on chromosome 19q13.4 represent the largest contiguous group of proteases within the human genome. Tissue kallikreins have attracted great interest as potential biomarkers for various cancers, including prostate, ovarian, breast, testicular, and lung (2, 3). Human Kallikrein 6 (hKLK6) is a member of tissue kallikrein family observed in breast and brain tissues, colon carcinoma cells, and oligodedrocytes (1-3). Known protein substrates of hKLK6 are myelin basic protein, the precursor of the A beta amyloid peptide, and plasminogen. Its physiological functions may include the participation in demyelination processes as well as in the progression of inflammatory disease of the CNS. The level of hKLK6 is reduced in brain extracts of Alzheimer patients and increased in serum of patients with ovarian cancer.

1. Emami, N. and E.P. Diamandis (2007) Clin. Chim. Acta 381:78.
2. Borgoño, C.A. and E.P. Diamandis (2004) Nat. Rev. Cancer 4:876.
3. Paliouras, M. et al. (2007) Cancer Lett. 249:61.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kallikrein 6/Neurosin Antibody (AF2008)(8)

Publications using AF2008

• Ford, NC;Benedeck, RE;Mattoon, MT;Peterson, JK;Mesler, AL;Veniaminova, NA;Gardon, DJ;Tsai, SY;Uchida, Y;Wong, SY; Hair follicles modulate skin barrier function bioRxiv : the preprint server for biology 2024-04-27 [PMID: 38712094] (Immunohistochemistry, Mouse)
• Hwudaurw Chen, Earlphia Sells, Ritu Pandey, Edward R. Abril, Chiu-Hsieh Hsu, Robert S. Krouse et al. Kallikrein 6 protease advances colon tumorigenesis via induction of the high mobility group A2 protein Oncotarget 2019-10-22 [PMID: 31692974]
• Johnston Andrew, Xing Xianying, Wolterink Liza et al. IL-1 and IL-36 are dominant cytokines in generalized pustular psoriasis. The Journal of Allergy and Clinical Immunology 1/1/2017 [PMID: 28043870]
• Jochen Hess Expression of Kallikrein-Related Peptidase 6 in Primary Mucosal Malignant Melanoma of the Head and Neck Head Neck Pathol, 2016-11-14;0(0):. 2016-11-14 [PMID: 27844409] (IHC, Human)
• Schrader C, Kolb M, Zaoui K, Flechtenmacher C, Grabe N, Weber K, Hielscher T, Plinkert P, Hess J Kallikrein-related peptidase 6 regulates epithelial-to-mesenchymal transition and serves as prognostic biomarker for head and neck squamous cell carcinoma patients. Mol Cancer, 2015-05-20;14(0):107. 2015-05-20 [PMID: 25990935] (IHC, Human)
• Munoz-Repeto I, Garcia M, Kamieniak M, Ramon Y Cajal T, Domingo S, Cazorla A, Garcia Donas J, Hernando Polo S, Garcia Sagredo J, Hernandez E, Lacambra C, Saez R, Robles L, Borrego S, Prat J, Palacios J, Benitez J Phenotypic characterization of hereditary epithelial ovarian cancer based on a tissue microarray study. Histol Histopathol, 2013-01-01;28(1):133-44. 2013-01-01 [PMID: 23233066] (IHC-P, Human)
• Chia-Yao Lee, David Marzan, Grace Lin, Steve Goodison, Steve Silletti alpha 2 Integrin-Dependent Suppression of Pancreatic Adenocarcinoma Cell Invasion Involves Ectodomain Regulation of Kallikrein-Related Peptidase-5 Journal of Oncology 2011-12-13 [PMID: 22203845]
• Chee J, Singh J, Naran A, Misso NL, Thompson PJ, Bhoola KD Novel expression of kallikreins, kallikrein-related peptidases and kinin receptors in human pleural mesothelioma. Biol. Chem., 2007-11-01;388(11):1235-42. 2007-11-01 [PMID: 17976017] (IHC-P, Human)

Review for Kallikrein 6/Neurosin Antibody (AF2008) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 01/06/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 20846516
• Species: Human

Bioinformatics

• Gene Symbol: KLK6
• Uniprot:
  • Q92876()