Neuropilin-1 Antibody [Allophycocyanin]

bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse/Rat Neuropilin-1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB566A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

PC-12 rat adrenal pheochromocytoma cell line was stained with Goat Anti-Mouse/Rat Neuropilin-1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB566A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu, Rt
• Applications: Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Allophycocyanin

Neuropilin-1 Antibody [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1
  Phe22-Ala810 (Lys811Arg), Ser829-Asp854
  Accession # Q9QWJ9
• Specificity: Detects mouse and rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 8% cross-reactivity with recombinant human Neuropilin-1 is observed, and less than 1% cross-reactivity with recombinant rat Neuropilin-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NRP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

* This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California. U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending. This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuropilin-1 Antibody [Allophycocyanin]

• BDCA4
• BDCA-4
• CD304 antigen
• CD304
• DKFZp686A03134
• DKFZp781F1414
• neuropilin 1
• Neuropilin1
• Neuropilin-1
• NP1
• NRP
• NRP1
• transmembrane receptor
• Vascular endothelial cell growth factor 165 receptor
• VEGF165R

Background

Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins. The amino acid sequence of rat Neuropilin-1 extracellular domain is 98% and 93% identical to that of mouse and human Neuropilin-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neuropilin-1 Antibody (FAB566A)(11)

Publications using FAB566A

• Julien Rossignol, Zakia Belaid, Guillemette Fouquet, Flavia Guillem, Rachel Rignault, Pierre Milpied et al. Neuropilin-1 cooperates with PD-1 in CD8+ T cells predicting outcomes in melanoma patients treated with anti-PD1 iScience 2022-06-17 [PMID: 35874918]
• Frédéric Van Gool, Michelle L.T. Nguyen, Maxwell R. Mumbach, Ansuman T. Satpathy, Wendy L. Rosenthal, Simone Giacometti et al. A Mutation in the Transcription Factor Foxp3 Drives T Helper 2 Effector Function in Regulatory T Cells Immunity 2019-02-01 [PMID: 30709738]
• D Kim, V Lee, T Dorsey, L Niklason, L Gui, G Dai Neuropilin-1 Mediated Arterial Differentiation of Murine Pluripotent Stem Cells Stem Cells Dev., 2018-03-13;0(0):. 2018-03-13 [PMID: 29415620] (Flow Cytometry, ICC, Mouse)
• FOXP3+ Tregs require WASP to restrain Th2-mediated food allergy J Clin Invest, 2016-09-19;0(0):. 2016-09-19 [PMID: 27643438] (Flow Cytometry, Human)
• Agnieszka M Kabat, Oliver J Harrison, Thomas Riffelmacher, Amin E Moghaddam, Claire F Pearson, Adam Laing et al. The autophagy gene Atg16l1 differentially regulates Treg and TH2 cells to control intestinal inflammation eLife 2016-02-24 [PMID: 26910010]
• Uma Chandrasekaran, Woelsung Yi, Sanjay Gupta, Chien-Huan Weng, Eugenia Giannopoulou, Yurii Chinenov et al. Regulation of Effector Treg Cells in Murine Lupus Arthritis & Rheumatology 2018-02-08 [PMID: 26816213]
• Ilseyar Akhmetzyanova, Gennadiy Zelinskyy, Elisabeth Littwitz-Salomon, Anna Malyshkina, Kirsten K. Dietze, Hendrik Streeck et al. CD137 Agonist Therapy Can Reprogram Regulatory T Cells into Cytotoxic CD4+ T Cells with Antitumor Activity The Journal of Immunology 2016-01-01 [PMID: 26608920]
• Ray A, Basu S, Miller N, Chan A, Dittel B An increase in tolerogenic dendritic cell and natural regulatory T cell numbers during experimental autoimmune encephalomyelitis in Rras-/- mice results in attenuated disease. J Immunol, 2014-04-25;192(11):5109-17. 2014-04-25 [PMID: 24771856] (Flow Cytometry, Mouse)
• Jeremiah J. Faith, Philip P. Ahern, Vanessa K. Ridaura, Jiye Cheng, Jeffrey I. Gordon Identifying Gut Microbe–Host Phenotype Relationships Using Combinatorial Communities in Gnotobiotic Mice Science Translational Medicine 2014-01-22 [PMID: 24452263]
• Lara Myers, Jara J. Joedicke, Aaron B. Carmody, Ronald J. Messer, George Kassiotis, Jaquelin P. Dudley et al. IL-2–Independent and TNF-alpha –Dependent Expansion of V beta 5+ Natural Regulatory T Cells during Retrovirus Infection The Journal of Immunology 2013-06-01 [PMID: 23645880]
• Xie J, Wu T, Xu K, Huang IK, Cleaver O, Huang CL Endothelial-specific expression of WNK1 kinase is essential for angiogenesis and heart development in mice. Am. J. Pathol., 2009-07-30;175(3):1315-27. 2009-07-30 [PMID: 19644017] (IHC-Fr, Mouse)

FAQs for Neuropilin-1 Antibody (FAB566A). (Showing 1 - 2 of 2 FAQs).

1. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
   • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.
2. What research areas can Neuropilin-1 antibodies be used in?
   • Neuropilin-1 products can be applied in the following research areas: Adaptive Immunity, Angiogenesis, Breast Cancer, Cancer, Cardiovascular Biology, Neuroscience, and Virology Bacteria and Parasites.

Bioinformatics

• Gene Symbol: NRP1
• Uniprot:
  • Q9QWJ9()