Neuroligin 3/NLGN3 Antibody

Western Blot: Neuroligin 3/NLGN3 Antibody [NB100-79866] - HEK293 overexpressing Neuregulin3 and probed with (mock transfection in first lane).

Western Blot: Neuroligin 3/NLGN3 Antibody [NB100-79866] - (0.1ug/ml) staining of Human Brain (Amygdala) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Neuroligin 3/NLGN3 Antibody Summary

• Description: Novus Biologicals Goat Neuroligin 3/NLGN3 Antibody (NB100-79866) is a polyclonal antibody validated for use in WB and ELISA. Anti-Neuroligin 3/NLGN3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RKDKRRQEPLRQPS corresponding to internal region according to NP_061850.2.
• Epitope: RKDKRRQEPLRQPS
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NLGN3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.05 - 0.2 ug/ml
• Application Notes: WB: Approx. 85-90 kDa band observed in human brain (amygdala) lysates (calculated MW of 91.6 kDa band according to NP_061850.2). In transfected HEK293 transiently expressing Neuregulin3 a band of approx. 98 kDa is observed. This band is not observed in the non-transfected HEK293.
• Control:

  Neuroligin 3/NLGN3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Neuroligin 3/NLGN3 Antibody

• ASPGX1
• AUTSX1
• Gliotactin homolog
• HNL3
• KIAA1480HNL3
• Neuroligin 3
• neuroligin-3
• NL3
• NLGN3

Background

This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. Mutations in this gene may be associated with autism and Asperger syndrome. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but their full length sequences have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neuroligin 3/NLGN3 Antibody (NB100-79866)(1)

Publication using NB100-79866

• Tabuchi K, Blundell J, Etherton MR et al. A neuroligin-3 mutation implicated in autism increases inhibitory synaptic transmission in mice. Science 2007-10-05 [PMID: 17823315]

Bioinformatics

• Gene Symbol: NLGN3
• Entrez:
  • 54413(Human)
  • 245537(Mouse)
  • 171297(Rat)
• Uniprot:
  • NP_061850.2(Human)