Neuroligin 3/NLGN3 Antibody (566205)

Western blot shows lysates of human brain (hippocampus) tissue. PVDF Membrane was probed with 2 µg/mL of Human Neuroligin 3/NLGN3 Monoclonal Antibody (Catalog # MAB60881) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Neuroligin 3/NLGN3 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 566205
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Neuroligin 3/NLGN3 Antibody (566205) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Neuroligin 3/NLGN3
  Gln38-Ser709
  Accession # NP_061850
• Specificity: Detects human Neuroligin 3/NLGN3 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) Neuroligin 3v2 and recombinant rat (rr) Neuroligin 3v4 is observed. No cross-reactivity with rhNeuroligin 2, rhNeuroligin 4, rrNeuroligin 1, 1v2, or 2v2 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NLGN3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuroligin 3/NLGN3 Antibody (566205)

• ASPGX1
• AUTSX1
• Gliotactin homolog
• HNL3
• KIAA1480HNL3
• Neuroligin 3
• neuroligin-3
• NL3
• NLGN3

Background

NLGN3 (Neuroligin 3; also gliotactin homolog) is a 110-114 kDa member of the type-B carboxyesterase/lipase family of proteins.  It is a neuronal transmembrane protein that forms Ca⁺⁺-dependent intercellular junctions with short beta -neurexin isoforms.  This seems to contribute to both glutamatergic and GABAergic synapse formation.  Mutations in NLGN3 are associated with a reduction in protein expression and the occurrence of autism.  Mature human NLGN3 is an 811 amino acid (aa) type I transmembrane protein.  It contains a 672 aa extracellular domain (ECD) (aa 38-709), plus a 118 aa cytoplasmic region.  The ECD possesses a nonfunctional carboxyesterase domain (aa 41-625).  Multiple splice variants exist.  There is a deletion of aa 153-172 that may also be accompanied by an alternative start site at Met118, and a deletion of aa 153-192 that may also be accompanied by a five Lys substitution for aa 554-848.  Over aa 39-709, human NLGN3 shares 99% aa identity with mouse NLGN3.        

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NLGN3
• Uniprot:
  • NP_061850()