Recombinant Human Neurexin 1 beta Fc Chimera Protein, CF

Images

 
There are currently no images for Neurexin 1 beta/NXRN1b (5268-NX).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human Neurexin 1 beta Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Biotinylated recombinant rat NLGN-1v2 immobilized on a streptavidin-coated plate at 1 µg/mL
(100 μL/well) can bind Recombinant Human Neurexin 1 beta /NXRN1b Fc Chimera with an apparent Kd <4 nM.
Source
Mouse myeloma cell line, NS0-derived human Neurexin 1 beta/NXRN1b protein
Human Neurexin 1 beta
(Ala51-Ser363)
Accession # NP_620072
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Ala51
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
NRXN1
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
59.9 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
85-95 kDa, reducing conditions
Publications
Read Publications using
5268-NX in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Neurexin 1 beta Fc Chimera Protein, CF

  • Neurexin 1 beta
  • NXR1B
  • NXRN1b

Background

The alpha and beta forms of Neurexins 1-3 are transmembrane neuronal glycoproteins which are transcribed from each of three NRXN genes that utilize alternate promoters. Like other Neurexins, the extracellular domain (ECD) of Neurexin 1 alpha contains six LNS domains interspersed with three EGF-like domains, while that of Neurexin 1 beta contains only the sixth LNS domain and no EGF-like domains (1 - 3). Mature human Neurexin 1 beta is a 70 kDa glycosylated protein with a 313 amino acid (aa) ECD and a 56 aa cytoplasmic domain that contains a motif for binding PDZ scaffolding proteins (2, 4, 5). Within comparable regions of the ECD, human Neurexin 1 beta shares 99% aa sequence identity with mouse and rat Neurexin 1 beta. It shares 42% and 68% aa sequence identity with the ECDs of human Neurexin 2 beta and 3 beta, respectively. Neurexin 1 beta isoforms are differentiated by a 30 aa insertion at alternative splice site 4 (SS4), numbered according to splice sites in the longer alpha Neurexins (4, 5). This recombinant protein does not include the SS4 insertion. Neurexin 1 beta is expressed presynaptically at neuronal contacts and interacts with postsynaptic Neuroligins 1-4. The presence of the SS4 insertion significantly weakens this interaction, particularly with Neuroligins 1 and 4 (6-12). Binding affinity is also dependent on the splicing pattern of the Neuroligin (7, 10, 11). Within the presynaptic terminal, Neurexin 1 beta is required for the recruitment of scaffolding proteins and synaptic vesicles (13). Its interaction with Neuroligins 1, 3, and 4 induces the development of glutamatergic postsynaptic terminals containing NMDA receptors, while its interaction with Neuroligin 2 promotes GABAergic postsynaptic development (10, 12, 14, 15). The presence of the SS4 insertion reduces the ability of Neurexin 1 beta to promote glutamatergic contacts but is required for the development of Neuroligin 2/GABAergic contact development (10, 12).
  1. Craig, A.M. and Y. Kang (2007) Curr. Opin. Neurobiol. 17:43.
  2. Lise, M.-F. and A. El-Husseini (2006) Cell. Mol. Life Sci. 63:1833.
  3. Dean, C. and T. Dresbach (2006) Trends Neurosci. 29:21.
  4. Rowen, L. et al. (2002) Genomics 79:587.
  5. Ushkaryov, Y.A. et al. (1992) Science 257:50.
  6. Ichtchenko, K. et al. (1995) Cell 81:435.
  7. Comoletti, D. et al. (2006) Biochemistry 45:12816.
  8. Ichtchenko, K. et al. (1996) J. Biol. Chem. 271:2676.
  9. Arac, D. et al. (2007) Neuron 56:992.
  10. Chih, B. et al. (2006) Neuron 51:171.
  11. Boucard, A.A. et al. (2005) Neuron 48:229.
  12. Graf, E.R. et al. (2006) J. Neurosci. 26:4256.
  13. Dean, C. et al. (2003) Nat. Neurosci. 7:708.
  14. Nam, C.I. and L. Chen (2005) Proc. Natl. Acad. Sci. 102:6137.
  15. Graf, E.R. et al. (2004) Cell 119:1013.

Customers Who Viewed This Item Also Viewed...

AF4340
Species: Hu, Rt
Applications: WB, IHC
AF5269
Species: Hu, Mu
Applications: WB, IHC
NBP1-90080
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-88182
Species: Hu
Applications: WB, IHC, IHC-P
AF5158
Species: Hu
Applications: WB, IHC
NBP2-43681
Species: Hu
Applications: WB, IP
NBP1-88769
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-41299
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-47610
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB100-74510
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP1-87486
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-58781
Species: Hu
Applications: IHC, IHC-P
NB300-556
Species: Hu, Mu, Rt, In, Pm
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, ICC
NBP2-00594
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NBP2-76922
Species: Hu, Rt
Applications: WB, DB, ELISA, Flow, ICC/IF, IHC, ICC
AF7035
Species: Hu
Applications: IHC
NB120-15855
Species: Hu, Mu, Rt, Bv
Applications: WB
MAB5416
Species: Hu
Applications: IHC
NBP2-13169
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP

Publications for Neurexin 1 beta/NXRN1b (5268-NX)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Immunoprecipitation, Neutralization.


Filter By Application
Immunoprecipitation
(1)
Neutralization
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species

Reviews for Neurexin 1 beta/NXRN1b (5268-NX) (0)

There are no reviews for Neurexin 1 beta/NXRN1b (5268-NX). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Neurexin 1 beta/NXRN1b (5268-NX) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Neurexin 1 beta/NXRN1b Products

Bioinformatics Tool for Neurexin 1 beta/NXRN1b (5268-NX)

Discover related pathways, diseases and genes to Neurexin 1 beta/NXRN1b (5268-NX). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Neurexin 1 beta/NXRN1b (5268-NX)

Discover more about diseases related to Neurexin 1 beta/NXRN1b (5268-NX).
 

Pathways for Neurexin 1 beta/NXRN1b (5268-NX)

View related products by pathway.

PTMs for Neurexin 1 beta/NXRN1b (5268-NX)

Learn more about PTMs related to Neurexin 1 beta/NXRN1b (5268-NX).

Blogs on Neurexin 1 beta/NXRN1b

There are no specific blogs for Neurexin 1 beta/NXRN1b, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Neurexin 1 beta Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol NRXN1
Uniprot