Neurogenin-2 Antibody (2A8)

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Western Blot: Neurogenin-2 Antibody (2A8) [H00063973-M10] - Analysis of NEUROG2 expression in H9c2 (2-1).

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, Single Cell Western
Clone
2A8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Neurogenin-2 Antibody (2A8) Summary

Immunogen
NEUROG2 (NP_076924, 74 a.a. ~ 175 a.a) full length recombinant protein with GST tag.CRPARLLGLVHDCKRRPSRARAVSRGAKTAETVQRIKKTRRLKANNRERNRMHNLNAALDALREVLPTFPEDAKLTKIETLRFAHNYIWALTETLRLADHCG
Specificity
NEUROG2 (2A8)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NEUROG2
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Single Cell Western 100 ug/ml
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.
Reviewed Applications
Read 1 Review rated 5
using
H00063973-M10 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Neurogenin-2 Antibody (2A8)

  • Atoh4
  • BHLHA8
  • bHLHa8MGC46562
  • Class A basic helix-loop-helix protein 8
  • Math4A
  • NEUROG2
  • neurogenin 2
  • Neurogenin2
  • Neurogenin-2
  • NGN2
  • ngn-2
  • Protein atonal homolog 4

Background

Neurogenin-2 is a member of the neurogenin subfamily of basic helix-loop-helix (bHLH) transcription factor genes that play an important role in neurogenesis from migratory neural crest cells.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neurogenin-2 Antibody (H00063973-M10) (0)

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Review for Neurogenin-2 Antibody (H00063973-M10) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using H00063973-M10:
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(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot Neurogenin-2 H00063973-M10
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reviewed by:
Anonymous
WB Other 04/01/2015
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Summary

ApplicationWestern Blot
Sample TestedRabbit Brain
SpeciesOther

Blocking

Blocking Details-

Details

Detection Notes-

Comments

CommentsAbout 30ug total protein lysate separated on 4-20% TGX gradient gel from Bio-Rad followed by transferring on PVDF membrane. 1ug/ml primary antibody Neurogenin-2 Cat # H00063973-M10 probed overnight in cold room in TBST. After washing 15minutes X 3 we probed secondary antibody 1/10000 goat anti Mouse at room temperature for 1 hour followed by washing 15minutes X 3. We used ECL prime to develop the film from GE health care.

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Secondary Antibodies

 

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
04/01/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol NEUROG2
Entrez
OMIM
Uniprot