Netrin-1 Antibody

Images

 
Western Blot: Netrin-1 Antibody [NBP1-51922] - Staining of Rat Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Netrin-1 Antibody [NBP1-51922] - Staining of paraffin embedded Human Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

Netrin-1 Antibody Summary

Immunogen
Peptide with sequence KFQQREKKGKCKKA corresponding to C-Terminus according to NP_004813.2.
Epitope
KFQQREKKGKCKKA
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NTN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.5 ug/ml
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 70 kDa band observed in human brain is reported in scientific literature (PMID: 24510764). IHC: Paraffin embedded Human Intestine.
Publications
Read Publication using NBP1-51922.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Netrin-1 Antibody

  • netrin 1
  • Netrin1
  • Netrin-1
  • NTN1
  • NTN1Lnetrin 1, mouse, homolog of

Background

Semaphorins, neuropilins and netrins are among a number of molecules and their receptors that regulate the developing nervous system to guide the development of neural circuits.1 Although first identified as axon guidance cues,2,3 it is now apparent that many of these same factors are not limited to the guidance of growing axons, but have roles in a range of processes from the guidance of cell migration to the regulation of the immune response, angiogenesis, lung branching morphogenesis, nervous system regeneration, and cancer.4-9 The semaphorins make up the largest family of axon guidance cues. They are characterized by the presence of an approximately 500 amino acid N-terminal semaphorin (Sema) domain. Semaphorins function mainly as chemorepellents that direct axons away from tissues.3 Semaphorin 3A (Sema3A) has been shown to be repellent to cortical axons and to inhibit axon branching.10 The transmembrane protein semaphorin 6A has been shown to repel embryonic sympathetic axons.11 The actions of the various semaphorins are not always similar. Semaphorin 3A has been found to inhibit tumor development whereas semaphorin 6A may contribute to tumor progression.9 Neuropilins are the ligand binding moieties in the class 3 Semaphorin receptor complexes that subsequently activate signaling through associated plexins. Two types have been identified so far: Neuropilin-1 (Npn-1) and Neuropilin-2 (Npn-2) receptors. At the amino acid sequence level, Npn-1 and Npn-2 share 44% identity. Npn-1 and Npn-2 show different expression patterns in developing neurons of the central and peripheral nervous systems, and show different binding specificities for different members of he semaphorin family. Both also function as receptors for some forms of vascular endothelial growth factor (VEGF).12 Netrins are a family of laminin-related small proteins that are involved in axon guidance and eurite outgrowth. Netrin-1 has been shown to attract cortical growth cones and promote axon branching.10 Netrin-4 (first named b-netrin) was found to promote neurite elongation from olfactory bulb explants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Netrin-1 Antibody (NBP1-51922)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NTN1
Entrez
Uniprot