Netrin-1 Antibody [Alexa Fluor® 647]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 647

Netrin-1 Antibody [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Netrin-1
  Val22-Ala604
  Accession # O95631
• Specificity: Detects human, mouse, and rat Netrin-1 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Netrin-1 Antibody [Alexa Fluor® 647]

• netrin 1
• Netrin1
• Netrin-1
• NTN1
• NTN1Lnetrin 1, mouse, homolog of

Background

Netrin-1 (from Sanskrit netr meaning "to guide"; also known as Epididymis tissue protein Li 131P) is a 66-76 kDa secreted member of the laminin superfamily of molecules. It is expressed by multiple cell types, including embryonic neural tube ventral midline cells plus postnatal renal tubule epithelium and oligodendroglia. Netrin-1 has multiple receptors, including beta 4-integrins, DCC, DSCAM and UNC5A-thru-D. Its effects are context specific. For example, on neurons, binding to either DCC or DSCAM alone promotes chemoattraction, while a DCC:UNC5 complex promotes chemorepulsion. In addition, the ligation of UNC5B on leukocytes suppresses cytokine production, while the binding of netrin to DCC expressed on select cell types inhibits apoptosis. Mature human netrin-1 is 580 amino acids (aa) in length (aa 25-604). It contains an N-terminal laminin domain (aa 47-284), three EGF-like domains (aa 285-453) and a C-terminal cell-interaction netrin-like domain (aa 472-601). Over aa 22-604, human netrin-1 shares 99% aa sequence identity with mouse netrin-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.