NET1 Antibody - BSA Free

Images

 
Western Blot: NET1 Antibody [NBP2-13653] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-380
Immunocytochemistry/ Immunofluorescence: NET1 Antibody [NBP2-13653] - Staining of human cell line HaCaT shows localization to nucleus & vesicles.
Immunohistochemistry-Paraffin: NET1 Antibody [NBP2-13653] - Staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

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Catalog# & Formulation Size Price

NET1 Antibody - BSA Free Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: PSARKLTAQRRASTVSSVTQVEVDENAYRCGSGMQMAEDSKSLKTHQTQPGIRRARDKALSGGKRKETL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NET1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NET1 Protein (NBP2-13653PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NET1 Antibody - BSA Free

  • ARHGEF8neuroepithelial cell-transforming gene 1 protein
  • guanine nucleotide regulatory protein (oncogene)
  • NET1A
  • neuroepithelial cell transforming 1
  • neuroepithelial cell transforming gene 1
  • neuroepithelioma transforming gene 1
  • p65 Net1 proto-oncogene protein
  • Proto-oncogene p65 Net1
  • Rho guanine nucleotide exchange factor (GEF) 8
  • Rho guanine nucleotide exchange factor 8
  • small GTP-binding protein regulator

Background

NET1 is a guanyl nucleotide exchange factor for RhoA GTPase. It may be involved in signal transduction, in the activation of the SAPK/JNK pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NET1