Nestin Antibody (196908) [Fluorescein]

NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human Nestin Fluorescein-conjugated Monoclonal Antibody (Catalog # IC1259F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 196908
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

Nestin Antibody (196908) [Fluorescein] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human Nestin
  Accession # P48681
• Specificity: Detects human Nestin in flow cytometry.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NES
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nestin Antibody (196908) [Fluorescein]

• FLJ21841
• NES
• Nestin

Background

Nestin is a class VI intermediate filament protein that is expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells. Its transient expression is a critical step in both the neural differentiation pathway and that of CNS glia. Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors and hematopoietic progenitors. Full-length human Nestin shares 51% amino acid sequence identity to mouse Nestin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nestin Antibody (IC1259F)(6)

Publications using IC1259F

• A Genchi, E Brambilla, F Sangalli, M Radaelli, M Bacigalupp, R Furlan, A Andolfo, D Drago, C Magagnotti, GM Scotti, R Greco, P Vezzulli, L Ottoboni, M Bonopane, D Capilupo, F Ruffini, D Belotti, B Cabiati, S Cesana, G Matera, L Leocani, V Martinelli, L Moiola, L Vago, P Panina-Bor, A Falini, F Ciceri, A Uglietti, MP Sormani, G Comi, MA Battaglia, MA Rocca, L Storelli, E Pagani, G Gaipa, G Martino Neural stem cell transplantation in patients with progressive multiple sclerosis: an open-label, phase 1 study Nature Medicine, 2023-01-09;29(1):75-85. 2023-01-09 [PMID: 36624312] (ICC, Human)
• S Mount, P Kanda, S Parent, S Khan, C Michie, L Davila, V Chan, RA Davies, H Haddad, D Courtman, DJ Stewart, DR Davis Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium Stem Cell Res Ther, 2019-11-04;10(1):316. 2019-11-04 [PMID: 31685023] (Flow Cytometry, Human)
• Azaz Ahmad, Stephanie Strohbuecker, Cristina Tufarelli, Virginie Sottile Expression of a SOX1 overlapping transcript in neural differentiation and cancer models Cellular and Molecular Life Sciences 2017-11-01 [PMID: 28674729]
• Annabelle Congras Non integrative strategy decreases chromosome instability and improves endogenous pluripotency genes reactivation in porcine induced pluripotent-like stem cells Sci Rep, 2016-06-01;6(0):27059. 2016-06-01 [PMID: 27245508] (Flow Cytometry, Porcine)
• Modeling Smith-Lemli-Opitz syndrome with induced pluripotent stem cells reveals a causal role for Wnt/?-catenin defects in neuronal cholesterol synthesis phenotypes Nat. Med., 2016-03-21;22(4):388-96. 2016-03-21 [PMID: 26998835] (Flow Cytometry, Human)
• Zhen Y, Zhao S, Li Q Arsenic trioxide-mediated Notch pathway inhibition depletes the cancer stem-like cell population in gliomas. Cancer Lett., 2009-12-04;292(1):64-72. 2009-12-04 [PMID: 19962820] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: NES
• Uniprot:
  • P48681()