Neprilysin/CD10 Antibody [Alexa Fluor® 488]

• Reactivity: Mu
• Applications: WB, IHC, IP, ELISA(Cap), ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

Neprilysin/CD10 Antibody [Alexa Fluor® 488] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Neprilysin/CD10
  Tyr52-Trp750
  Accession # AAA37386
• Specificity: Detects mouse Neprilysin/CD10 in ELISAs and Western blots. In sandwich immunoassays, less than 35% cross-reactivity with recombinant human (rh) Neprilysin is observed and less than 0.2% cross-reactivity with rhNeprilysin-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neprilysin/CD10 Antibody [Alexa Fluor® 488]

• Atriopeptidase
• CALLA
• CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase)
• CD10 antigen
• CD10
• CD10)
• CD10membrane metallo-endopeptidase variant 1
• Common acute lymphocytic leukemia antigen
• DKFZp686O16152
• EC 3.4.24
• EC 3.4.24.11
• Enkephalinase
• EPN
• Leu-19
• membrane metallo-endopeptidase variant 2
• membrane metallo-endopeptidase
• MGC126681
• MGC126707
• MME
• NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA
• Neprilysin
• Neutral Endopeptidase 24.11
• Neutral endopeptidase
• NKH1
• SFE
• Skin fibroblast elastase

Background

Neprilysin (NEP, neutral endopeptidase 24.11, EC 3.4.24.11) is a zinc metallopeptidase expressed at the cell surface of a variety of cells. The enzyme functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. NEP has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide (1, 2). NEP has also been identified as the common acute lymphoblastic leukemia antigen (CALLA), and to be expressed on the surface of lymphocytes in some disease states (3). These and other observations have resulted in considerable clinical interest in NEP as a potential target for analgesics and antihypertensive drugs. NEP is also a major degrading enzyme of amyloid beta peptide (A beta ) in the brain, indicating that down-regulation of NEP activity, which could be caused by aging, can contribute to the development of Alzheimer’s disease by promoting A beta accumulation (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • AAA37386()