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VCTS021). Tissue was stained using the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">
VCTS021). Tissue was stained using the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">
DY990). Goat Anti-Mouse Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1126) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
AF1126) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1126) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
HAF109). For additional reference, Recombinant Mouse Neprilysin (1126-ZN) (5 and 50 ng/lane) and Recombinant Human Neprilysin (CHO-expressed) (1182-ZNC) (5 and 50 ng/lane) were included. Specific bands were detected for Neprilysin/CD10 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
1182-ZNC) was serially diluted and captured by Goat Anti-Human Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1182) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1182) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
1182-ZNC) was serially diluted and captured by Goat Anti-Human Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1182) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1182) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules and glomeruli. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
HAF005). For additional reference, Recombinant Mouse Neprilysin (Catalog # 1126-ZN) (5 and 50 ng/lane) and Recombinant Human Neprilysin (CHO-expressed) (Catalog # 1182-ZNC) (5 and 50 ng/lane) were included. Specific bands were detected for Neprilysin/CD10 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
CD10, also known as the Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.
![Immunohistochemistry Neprilysin/CD10 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Neprilysin_AF1126_Immunohistochemistry_6679.jpg)
![Immunohistochemistry Neprilysin/CD10 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Neprilysin_AF1182_Immunohistochemistry_17341.jpg)
![Immunohistochemistry Neprilysin/CD10 Antibody (200103) [Unconjugated]](http://images.novusbio.com/images/antibody/Neprilysin_MAB1126_Immunohistochemistry_22731.jpg)
![N/A Neprilysin/CD10 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Neprilysin_DY1182_ELISA_1594.jpg)
![N/A Neprilysin/CD10 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Neprilysin_DY1126_ELISA_1579.jpg)
![Western Blot Neprilysin/CD10 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1126_mouse-neprilysin-affinity-purified-polyclonal-ab-8220249505976.jpg)
![Western Blot Neprilysin/CD10 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1126_mouse-neprilysin-affinity-purified-polyclonal-ab-44202415433122.jpg)
![Immunohistochemistry Neprilysin/CD10 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af1126_mouse-neprilysin-affinity-purified-polyclonal-ab-immunohistochemistry-7520268331.png)
![N/A Neprilysin/CD10 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af1126_mouse-neprilysin-affinity-purified-polyclonal-ab-7520268433.png)
![ELISA Neprilysin/CD10 Antibody [Unconjugated]](http://images.novusbio.com/images/af1126_mouse-neprilysin-affinity-purified-polyclonal-ab-elisa-639201740030075101.jpg)
![ELISA Neprilysin/CD10 Antibody [Unconjugated]](http://images.novusbio.com/images/af1126_mouse-neprilysin-affinity-purified-polyclonal-ab-elisa-639201854770313959.jpg)
![Western Blot Neprilysin/CD10 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Neprilysin_AF1182_Western_Blot_11005.jpg)
![Western Blot Neprilysin/CD10 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1182_human-neprilysin-affinity-purified-polyclonal-ab-812025556133.jpg)
![ELISA Neprilysin/CD10 Antibody [Unconjugated]](http://images.novusbio.com/images/af1182_human-neprilysin-affinity-purified-polyclonal-ab-elisa-639201740030760625.jpg)
![ELISA Neprilysin/CD10 Antibody [Unconjugated]](http://images.novusbio.com/images/af1182_human-neprilysin-affinity-purified-polyclonal-ab-elisa-639201854770404764.jpg)
![Western Blot Neprilysin/CD10 Antibody (200103) [Unconjugated]](http://images.novusbio.com/images/antibody/Neprilysin_MAB1126_Western_Blot_11004.jpg)
![Western Blot Neprilysin/CD10 Antibody (200103) [Unconjugated]](http://images.novusbio.com/fullsize/mab1126_human-mouse-neprilysin-ectodomain-mab-clone-200103-8120255541740.jpg)
![Enzyme Activity Neprilysin/CD10 [Unconjugated]](http://images.novusbio.com/images/10834-zn_recombinant-cynomolgu-rhesus-neprilysin-cd10-his-protein-cf-enzyme-activity-2852021102023.jpg)
![SDS-Page Neprilysin/CD10 [Unconjugated]](http://images.novusbio.com/fullsize/10834-zn_recombinant-cynomolgu-rhesus-neprilysin-cd10-his-protein-cf-sds-page-285202194934.jpg)
