Nephrin Antibody (1037720) [Unconjugated]

Western blot shows lysates of HEK293T human embryonic kidney cell line and human kidney (medulla). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Nephrin Monoclonal Antibody (Catalog # MAB42692) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Nephrin at approximately 134 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 1037720
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Nephrin Antibody (1037720) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived human Nephrin
  Gln23-Thr1029
  Accession # O60500
• Specificity: Detects human Nephrin in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nephrin Antibody (1037720) [Unconjugated]

• CNF
• Nephrin
• nephrosis 1, congenital, Finnish type (nephrin)
• NPHNCNF
• NPHS1
• Renal glomerulus-specific cell adhesion receptor

Background

Nephrin (or renal glomerulus-specific cell adhesion receptor; also NPHS1) is a 185 - 200 kDa member of the Ig superfamily. It is expressed on glomerular podocyte membranes, associates with same-cell Neph1, and forms homomeric zipper-like complexes with Nephrin on adjacent podocytes. Mature human Nephrin is a type I transmembrane glycoprotein 1219 amino acids (aa) in length. It contains a 1033 aa extracellular region and a 165 aa cytoplasmic tail. One potential, soluble splice form is known where aa’s 1056 - 1095 are deleted, eliminating the transmembrane region. Over aa 23 - 1029, human Nephrin shares 84% and 89% aa identity with mouse and dog Nephrin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nephrin Antibody (MAB42692)(1)

Publication using MAB42692

• Liu, P;Liu, S;Dalal, V;Lane, J;Gessaroli, E;Forte, E;Gallon, L;Jin, J; Evaluation of Methodologies in Anti-nephrin Autoantibody Detection bioRxiv : the preprint server for biology 2024-07-25 [PMID: 39211159] (ELISA Capture, Human)

Bioinformatics

• Uniprot:
  • O60500()