Nectin-2/CD112 Antibody (110) - Azide and BSA Free

Western Blot: Nectin-2/CD112 Antibody (110) [NBP2-90530] - Anti-Carbonic Anhydrase XIV/CA14 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Membrane Lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa

Immunoprecipitation: Nectin-2/CD112 Antibody (110) [NBP2-90530] - Mouse Nectin-2/CD112 was immunoprecipitated using: Lane A: 0.5 mg Raji Whole Cell Lysate. 0.5 ul anti-Mouse Nectin-2/CD112 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Nectin-2/CD112 rabbit monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 58 kDa. Observed band size: 58 kDa

• Reactivity: Mu
• Applications: WB, ELISA, IP
• Clone: 110
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Nectin-2/CD112 Antibody (110) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Nectin-2/CD112 (Accession#: NP_033016.3; Met 1-Gly 351).
• Specificity: No cross-reactivity in ELISA with:
  
  Human CD112 / Nectin-2
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NECTIN2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunoprecipitation 0.2-1 uL/mg of lysate
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Nectin-2/CD112 Antibody (110) - Azide and BSA Free

• CD112 antigen
• CD112
• Herpes virus entry mediator B
• Herpesvirus entry mediator B
• herpesvirus entry protein B
• HVEB
• HVEBpoliovirus receptor-like 2
• MPH
• nectin 2
• Nectin2
• Nectin-2
• poliovirus receptor-related 2 (herpesvirus entry mediator B)
• poliovirus receptor-related protein 2
• PRR2
• PRR2nectin-2
• PVRL2
• PVRR2poliovirus receptor related 2

Background

Nectin 2 encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NECTIN2