Nectin-1/PVRL1 Antibody (R1.302) - BSA Free

Flow (Cell Surface): Nectin-1/PVRL1 Antibody (R1.302) [NBP2-54643] - Surface staining of human peripheral blood with anti-human CD111 (R1.302) PE.

Flow Cytometry: Nectin-1/PVRL1 Antibody (R1.302) [NBP2-54643] - Surface staining of human peripheral blood with anti-human CD111 (R1.302) purified, GAM-APC.

• Reactivity: Hu
• Applications: WB, Flow, Flow-CS, ICC/IF, IHC, IP, CyTOF-ready
• Clone: R1.302
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Nectin-1/PVRL1 Antibody (R1.302) - BSA Free Summary

• Immunogen: NIH/3T3 cells transfected with human CD111
• Specificity: The mouse monoclonal antibody R1.302 recognizes CD111 (also known as Nectin 1), a 75 kDa type I transmembrane glycoprotein broadly expressed on endothelial cells, epithelial cells, neuronal cells, megakaryocytes, and CD34-positive stem cells.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NECTIN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Cell Surface) 1:10-1:1000
  • Flow Cytometry 1-4 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Nectin-1/PVRL1 Antibody (R1.302) - BSA Free

• CD111 antigen
• CD111
• CLPED1ectodermal dysplasia 4 (Margarita Island type)
• ED4
• Herpes virus entry mediator C
• Herpesvirus entry mediator C
• Herpesvirus Ig-like receptor
• HIgRPVRR
• HVEC
• HVECpoliovirus receptor-like 1
• MGC142031
• Nectin1
• Nectin-1
• OFC7nectin 1
• poliovirus receptor-related 1 (herpesvirus entry mediator C)
• poliovirus receptor-related protein 1
• PRR
• PRR1
• PRR1MGC16207
• PVRL1
• PVRR1
• PVRR1nectin-1
• SK-12

Background

CD111, also known as nectin-1, is a calcium-independent cell-cell adhesion transmembrane glycoprotein involved in organization of adherens junctions and tight junctions in epithelial and endothelial cells. It also serves as a target molecule for entry of herpes simplex virus (HSV-1, HSV-2) and pseudorabies virus (PRV) into epithelial and neuronal cells. CD111 is connected with actin cytoskeleton through afadin. Mutations in the gene for CD111 cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NECTIN1