Nectin-1/PVRL1 Antibody (610835) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
610835
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 594
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Nectin-1/PVRL1 Antibody (610835) [Alexa Fluor® 594] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Nectin-1
Gln31-Thr334
Accession # Q15223
Specificity
Detects human Nectin-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Nectin-2, 3, 4, or recombinant mouse Nectin-1 is observed.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PVRL1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nectin-1/PVRL1 Antibody (610835) [Alexa Fluor® 594]

  • CD111 antigen
  • CD111
  • CLPED1ectodermal dysplasia 4 (Margarita Island type)
  • ED4
  • Herpes virus entry mediator C
  • Herpesvirus entry mediator C
  • Herpesvirus Ig-like receptor
  • HIgRPVRR
  • HVEC
  • HVECpoliovirus receptor-like 1
  • MGC142031
  • Nectin1
  • Nectin-1
  • OFC7nectin 1
  • poliovirus receptor-related 1 (herpesvirus entry mediator C)
  • poliovirus receptor-related protein 1
  • PRR
  • PRR1
  • PRR1MGC16207
  • PVRL1
  • PVRR1
  • PVRR1nectin-1
  • SK-12

Background

Nectin-1 (designated CD111), also called PRR-1 (poliovirus receptor-related protein 1) or HVEC (herpesvirus entry mediator C), is a widely expressed 110 kDa type I transmembrane glycoprotein important in formation of adherens junctions and synapses. It is a member of the nectin family within the Ig superfamily (1, 2). The Latin word necto means “to connect”, indicating the role of nectins in Ca2+-independent cell-cell adhesion (2). Nectin-1 forms homodimers in cis, followed by interactions in trans with Nectin-1, -3 or -4 (2). The 517 amino acid (aa) human Nectin-1 isoform 1 contains a 30 aa signal sequence, a 325 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 141 aa cytoplasmic region. Nectin ECDs contain three Ig-like domains: an N-terminal V-type that mediates ligand binding and two C2-type (3). Nectin-1, like other nectins, has a splice form (isoform 2 or HigR, 458 aa) with alternate TM and cytoplasmic sequences. Another, isoform 3, is a 352 aa secreted protein (4). The common region of mature human Nectin-1 (aa 31-334) shares 93%, 94%, 96% and 96% aa identity with mouse, rat, bovine and porcine Nectin-1, respectively. Nectin-1 binds viral glycoprotein D to mediate herpesvirus (but not poxvirus) entry into vaginal mucosa, sensory neurons and fibroblasts (4 - 7). In forming adherens junctions and synapses, nectins 1 and 3 initiate cell-cell interactions, recruiting alpha v beta 3 integrin extracellularly and cadherins intracellularly through afadin and other junctional proteins (2, 8 - 11). These interactions organize the cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. Nectin-1 also recognizes CD96 on NK cells (12). Deficiency of Nectin-1 can result in cleft lip/palate ectodermal dysplasia (13). Nectin-1 downregulation in epithelial cancers, mediated in part by ectodomain shedding, may contribute to invasiveness (14).

  1. Lopez, M. et al. (1995) Gene 155:261.
  2. Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
  3. Fabre, S. et al. (2002) J. Biol. Chem. 277:27006.
  4. Lopez, M. et al. (2001) J. Virol. 75:5684.
  5. Cocchi, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15700.
  6. Linehan, M. M. et al. (2004) J. Virol. 78:2530.
  7. Simpson, S. A. et al. (2005) J. Neurovirol. 11:208.
  8. Mizoguchi, A. et al. (2002) J. Cell Biol. 156:555.
  9. Togashi, H. et al. (2006) J. Cell Biol. 174:141.
  10. Tachibana, K. et al. (2000) J. Cell Biol. 150:1161.
  11. Takai, Y. and H. Nakanishi (2003) J. Cell Science 116:17.
  12. Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
  13. Suzuki, K. et al. (2000) Nat. Genet. 25:427.
  14. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB2880U
Alexa Fluor 405 FAB2880V
Alexa Fluor 488 FAB2880G
Alexa Fluor 594 FAB2880T
Alexa Fluor 647 FAB2880R
Alexa Fluor 700 FAB2880N
Alexa Fluor 750 FAB2880S

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Bioinformatics

Gene Symbol PVRL1
Uniprot