Nectin-1/PVRL1 Antibody (610835) [Alexa Fluor™ Plus 488]

• Clone: 610835
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 488

Nectin-1/PVRL1 Antibody (610835) [Alexa Fluor™ Plus 488] Summary

• Specificity: Detects human Nectin-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Nectin-2, 3, 4, or recombinant mouse Nectin-1 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Nectin-1 (designated CD111), also called PRR-1 (poliovirus receptor-related protein 1) or HVEC (herpesvirus entry mediator C), is a widely expressed 110 kDa type I transmembrane glycoprotein important in formation of adherens junctions and synapses. It is a member of the nectin family within the Ig superfamily (1, 2). The Latin word necto means “to connect”, indicating the role of nectins in Ca²⁺-independent cell-cell adhesion (2). Nectin-1 forms homodimers in cis, followed by interactions in trans with Nectin-1, -3 or -4 (2). The 517 amino acid (aa) human Nectin-1 isoform 1 contains a 30 aa signal sequence, a 325 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 141 aa cytoplasmic region. Nectin ECDs contain three Ig-like domains: an N-terminal V-type that mediates ligand binding and two C2-type (3). Nectin-1, like other nectins, has a splice form (isoform 2 or HigR, 458 aa) with alternate TM and cytoplasmic sequences. Another, isoform 3, is a 352 aa secreted protein (4). The common region of mature human Nectin-1 (aa 31-334) shares 93%, 94%, 96% and 96% aa identity with mouse, rat, bovine and porcine Nectin-1, respectively. Nectin-1 binds viral glycoprotein D to mediate herpesvirus (but not poxvirus) entry into vaginal mucosa, sensory neurons and fibroblasts (4 - 7). In forming adherens junctions and synapses, nectins 1 and 3 initiate cell-cell interactions, recruiting alpha _v beta ₃ integrin extracellularly and cadherins intracellularly through afadin and other junctional proteins (2, 8 - 11). These interactions organize the cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. Nectin-1 also recognizes CD96 on NK cells (12). Deficiency of Nectin-1 can result in cleft lip/palate ectodermal dysplasia (13). Nectin-1 downregulation in epithelial cancers, mediated in part by ectodomain shedding, may contribute to invasiveness (14).

1. Lopez, M. et al. (1995) Gene 155:261.
2. Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
3. Fabre, S. et al. (2002) J. Biol. Chem. 277:27006.
4. Lopez, M. et al. (2001) J. Virol. 75:5684.
5. Cocchi, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15700.
6. Linehan, M. M. et al. (2004) J. Virol. 78:2530.
7. Simpson, S. A. et al. (2005) J. Neurovirol. 11:208.
8. Mizoguchi, A. et al. (2002) J. Cell Biol. 156:555.
9. Togashi, H. et al. (2006) J. Cell Biol. 174:141.
10. Tachibana, K. et al. (2000) J. Cell Biol. 150:1161.
11. Takai, Y. and H. Nakanishi (2003) J. Cell Science 116:17.
12. Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
13. Suzuki, K. et al. (2000) Nat. Genet. 25:427.
14. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.