NDUFB7 Antibody - BSA Free

Western Blot: NDUFB7 Antibody [NBP2-97854] - Anti-NDUFB7 rabbit polyclonal antibody at 1:500 dilution. Lane A: PC3 Whole Cell Lysate Lane B: HL60 Whole Cell Lysate Lane C: A431 Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 19 kDa

Immunocytochemistry/Immunofluorescence: NDUFB7 Antibody [NBP2-97854] - Immunofluorescence staining of NDUFB7 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human NDUFB7 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Immunohistochemistry-Paraffin: NDUFB7 Antibody [NBP2-97854] - Immunochemical staining of human NDUFB7 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: NDUFB7 Antibody [NBP2-97854] - Immunochemical staining of human NDUFB7 in cynomolgus heart with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: NDUFB7 Antibody [NBP2-97854] - NDUFB7 was immunoprecipitated using: Lane A: 0.5 mg HL60 Whole Cell Lysate 4 ul anti-NDUFB7 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NDUFB7 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 20 kDa

• Reactivity: Hu, Pm-Cm
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NDUFB7 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human NDUFB7 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDUFB7
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for NDUFB7 Antibody - BSA Free

• B18
• Cell adhesion protein SQM1
• CI-B18NADH-ubiquinone oxidoreductase B18 subunit
• complex I B18 subunit
• Complex I-B18
• MGC2480
• NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 (18kD, B18)
• NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa
• NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

Background

NDUFB7 is encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It is located at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDUFB7