NDUFA9 Antibody - BSA Free

Western Blot: NDUFA9 Antibody [NBP3-13135] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with NDUFA9 antibody (NBP3-13135) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: NDUFA9 Antibody [NBP3-13135] - NDUFA9 antibody detects NDUFA9 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: NDUFA9 stained by NDUFA9 antibody (NBP3-13135) diluted at 1:500. Blue: Hoechst 33342 staining.

Western Blot: NDUFA9 Antibody [NBP3-13135] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDUFA9 antibody (NBP3-13135) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Pm
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NDUFA9 Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NDUFA9. The exact sequence is proprietary.
• Predicted Species: Chimpanzee (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDUFA9
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot

Reactivity Notes

Mouse (81%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for NDUFA9 Antibody - BSA Free

• CC6
• CI39k
• CI-39k
• CI-39kD
• complex I 39kDa subunit
• Complex I-39kD
• MGC111043
• NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9 (39kD)
• NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa
• NADH dehydrogenase (ubiquinone) Fe-S protein 2-like (NADH-coenzyme Q reductase)
• NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial
• NADH-ubiquinone oxidoreductase 39 kDa subunit
• NDUFS2L
• SDR22E1
• short chain dehydrogenase/reductase family 22E, member 1

Background

ATP is generated via oxidative phosphorylation (Oxphos) in the mitochondria of almost all cells. The protein components of Oxphos, 4 respiratory chain complexes (I-IV) and an ATP synthase, are encoded in both the mitochondrial and nuclear genomes. Of the 4 respiratory chain complexes, complex I is the largest at 900 kD and contains at least 41 polypeptide subunits, 7 of which are encoded in the mitochondria, the remaining subunits are encoded in the nucleus. The multisubunit NADH:ubiquinone oxidoreductase is the first enzyme complex. By use of chaotropic agents, complex I can be fragmented into 3 different fractions. The flavoprotein fraction contains the NDUFV1, NDUFV2, and NDUFV3 subunits. The iron-sulfur protein (IP) fraction contains at least 7 subunits, NDUFS1-NDUFS6 and NDUFA5. The remaining subunits are part of the hydrophobic protein (HP) fraction. NDUFA9 is part of the hydrophobic protein fraction of the enzyme complex. However, it is predominantly hydrophilic, and appears to lie mostly outside the lipid bilayer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDUFA9