NDRG2 Antibody

Western Blot: NDRG2 Antibody [NBP1-72052] - (0.1ug/ml) staining of Human Temporal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-72052] - 3.75ug/ml staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: NDRG2 Antibody [NBP1-72052] - Staining of Mouse (A) and Rat (B) Brain lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NDRG2 Antibody Summary

• Immunogen: Peptide with sequence KEAELAARILLDQGQ-C corresponding to N-Terminus (near) according to NP_963293.1.
• Specificity: This antibody is expected to recognize reported isoform a (NP_963293.1) only. Reported variants represent identical protein: NP_963831.1, NP_963293.1, NP_963834.1, NP_963833.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NDRG2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.1-0.3ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1:1000
• Application Notes: Western blot: Approx 45kDa band observed in Human Brain (Temporal Cortex), Mouse and Rat Brain lysates (calculated MW of 40.8kDa according to NP_963293.1). Primary incubation was 1 hour
  IHC: Paraffin embedded Human Adrenal Gland.

Reactivity Notes

Expected from sequence similarity: Canine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NDRG2 Antibody

• DKFZp781G1938
• FLJ25522
• KIAA1248cytoplasmic protein Ndr1
• NDR1-related protein NDR2
• NDRG family member 2
• protein NDRG2
• Protein Syld709613
• syld709613 protein
• SYLDN-myc downstream regulator 2

Background

NDRG2 is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may play a role in neurite outgrowth. This gene may be involved in glioblastoma carcinogenesis. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NDRG2 Antibody (NBP1-72052)(1)

Publication using NBP1-72052

• Shi H, Li N, Li S et al. Expression of NDRG2 in esophageal squamous cell carcinoma. Cancer Sci 2010-05-01 [PMID: 20331630]

Bioinformatics

• Gene Symbol: NDRG2
• Entrez:
  • 57447(Human)
  • 29811(Mouse)
  • 171114(Rat)
• Uniprot:
  • NP_963293.1(Human)