NDRG2 Antibodies
We offer NDRG2 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Sandwich ELISA, Western Blot. Each NDRG2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our NDRG2 Antibodies can be used in a variety of model species: Bovine, Canine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat. Use the list below to choose the NDRG2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our NDRG2 polyclonal antibodies and browse our NDRG2 monoclonal antibody catalog.
Alternate Names for NDRG2 Antibodies
anti-NDRG2 antibody, anti-DKFZp781G1938 antibody, anti-FLJ25522 antibody, anti-KIAA1248cytoplasmic protein Ndr1 antibody, anti-NDR1-related protein NDR2 antibody, anti-NDRG family member 2 antibody, anti-protein NDRG2 antibody, anti-Protein Syld709613 antibody, anti-syld709613 protein antibody, anti-SYLDN-myc downstream regulator 2 antibody
9 Results for "ndrg2" in Primary Antibodies
![Western Blot: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 downregulates GLUT1 by promoting its ubiquitination. ((B), (D) and (F) T-47D cells were transfected with NDRG2 small interfering RNA (siRNA) 10, 25 and 100 pmol or control siRNA for 48 hours. Next, cell proteins or mRNA were extracted and analysed by immunoblotting (B). beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NDRG2-Antibody-6A5-Western-Blot-H00057447-M03-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 downregulates GLUT1 by promoting its ubiquitination. ((B), (D) and (F) T-47D cells were transfected with NDRG2 small interfering RNA (siRNA) 10, 25 and 100 pmol or control siRNA for 48 hours. Next, cell proteins or mRNA were extracted and analysed by immunoblotting (B). beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 interacts with GLUT1. SK-BR-3 cells were fixed and incubated with primary antibodies against N-myc downstream-regulated gene 2 (NDRG2) or glucose transporter 1 (GLUT1) and with fluorescein isothiocyanate or a cyanine 3 secondary antibody. Green fluorescence indicates NDRG2 expression, red fluorescence indicates GLUT1 expression and blue fluorescence indicates nuclear staining. The results of the merged images reveal that NDRG2 and GLUT1 were colocalised in the cytoplasm. Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NDRG2-Antibody-6A5-Immunocytochemistry-Immunofluorescence-H00057447-M03-img0005.jpg)
![Immunohistochemistry: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 is correlated with increased survival and negatively correlated with GLUT1 in breast carcinoma. Kaplan-Meier analysis was carried out according to N-myc downstream-regulated gene 2 (NDRG2) expression levels of disease-free survival. Serial immunostained sections for NDRG2 and glucose transporter 1 (GLUT1) in breast cancer and normal tissues were analysed. Original magnification, 40x (top) and 400x (bottom); scale bars = 50 um. Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NDRG2-Antibody-6A5-Immunohistochemistry-H00057447-M03-img0007.jpg)
![Western Blot: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 is correlated with increased survival and negatively correlated with GLUT1 in breast carcinoma. Kaplan-Meier analysis was carried out according to N-myc downstream-regulated gene 2 (NDRG2) expression levels of disease-free survival. Protein was extracted from matched breast tumour tissue (T) and adjacent normal tissue (N) and subjected to immunoblot analysis to examine NDRG2 and GLUT1 expression. beta-actin served as a loading control. P: patient. Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NDRG2-Antibody-6A5-Western-Blot-H00057447-M03-img0008.jpg)
![Western Blot: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 inhibits cell proliferation and reduces the intracellular glucose levels of breast cancer cells. T-47D, MCF-7, Bcap37, MDA-MB-231 and SK-BR-3 cells were collected for the extraction of proteins and analysed for N-myc downstream-regulated gene 2 (NDRG2) expression by immunoblotting. Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NDRG2-Antibody-6A5-Western-Blot-H00057447-M03-img0009.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 inhibits cell proliferation and reduces the intracellular glucose levels of breast cancer cells. Con siRNA. SK-BR-3 cells were cultured to glucose medium at concentrations of 0, 5, 25, 50 and 100 mM for 24 hours, and then the protein or mRNA was extracted for analysis by immunoblotting (E). beta-actin was used as a loading control. The data presented are means +/- SD; error bars represented SD from 3 replicative wells. *P < 0.05 and **P < 0.01 versus control group. Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NDRG2-Antibody-6A5-Western-Blot-H00057447-M03-img0011.jpg)
![Western Blot: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 inhibits cell proliferation and reduces the intracellular glucose levels of breast cancer cells. SK-BR-3 cells with low NDRG2 expression were infected by an adenovirus carrying NDRG2 (Ad-NDRG2) or negative control LacZ (Ad-LacZ), and T-47D cells with high NDRG2 were transfected with small interfering RNA targeting NDRG2 (NDRG2 siRNA) or negative control siRNA (Con siRNA). Thereafter proteins were extracted from these cells and analysed by immunoblotting. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NDRG2-Antibody-6A5-Western-Blot-H00057447-M03-img0010.jpg)
![Western Blot: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 interacts with GLUT1. Immunoprecipitation (IP) assays were performed with whole-cell lysates of SK-BR-3 cells pretreated with protein A-conjugated sepharose beads. Whole-cell lysates were probed for input. The antibodies for immunoprecipitation and Western blot (WB) analyses were carried out as indicated. The locations of various proteins are indicated by arrowheads. IgG, Immunoglobulin G. Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NDRG2-Antibody-6A5-Western-Blot-H00057447-M03-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 decreases the glucose uptake and GLUT1 protein levels in SK-BR-3-based subcutaneously xenograft tumours. The experiments illustrated are described in the Methods section. Intratumoural protein expression was assessed by N-myc downstream-regulated gene 2 (NDRG2) and glucose transporter 1 (GLUT1) IHC staining. Representative images are shown. Original magnification: 400 x; Scale bars = 50 um. Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NDRG2-Antibody-6A5-Immunocytochemistry-Immunofluorescence-H00057447-M03-img0006.jpg)
![Western Blot: NDRG2 Antibody (6A5) [H00057447-M03] - Analysis of NDRG2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/NDRG2-Antibody-6A5-Western-Blot-H00057447-M03-img0003.jpg)
![Western Blot: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 downregulates GLUT1 by promoting its ubiquitination. SK-BR-3 cells were infected with an adenovirus carrying N-myc downstream-regulated gene 2 (Ad-NDRG2) at 1, 5 and 10 multiplicity of infection (MOI) or Ad-LacZ for 48 hours. Next, cell proteins or mRNA were extracted and analysed by immunoblotting (A). beta-actin was used as a loading control. WB, Western blot. Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NDRG2-Antibody-6A5-Western-Blot-H00057447-M03-img0012.jpg)
![Western Blot: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 decreases the glucose uptake and GLUT1 protein levels in SK-BR-3-based subcutaneously xenograft tumours. The experiments illustrated are described in the Methods section. Proteins of the xenograft tumours from each group were extracted and analysed by immunoblotting to quantify NDRG2 and GLUT1 protein changes. Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NDRG2-Antibody-6A5-Western-Blot-H00057447-M03-img0015.jpg)
![ELISA: NDRG2 Antibody (6A5) [H00057447-M03] - Detection limit for recombinant GST tagged NDRG2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NDRG2-Antibody-6A5-ELISA-H00057447-M03-img0002.jpg)
![Western Blot: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 inhibits cell proliferation & reduces intracellular glucose levels of breast cancer cells. (E) & (F) SK-BR-3 cells cultured to glucose medium at concentrations of 0, 5, 25, 50 & 100 mM for 24 hrs, & then the protein or mRNA was extracted for analysis by immunoblotting (E) or real-time PCR (F). beta -actin used as a loading control. The data presented means ± SD; error bars represented SD from 3 replicative wells. *P < 0.05 & **P < 0.01 versus control group. Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00057447-m03_mouse-monoclonal-ndrg2-antibody-6a5-310202415304260.jpg)
![Western Blot: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 inhibits cell proliferation & reduces intracellular glucose levels of breast cancer cells. (A) T-47D, MCF-7, Bcap37, MDA-MB-231 & SK-BR-3 cells collected for the extraction of proteins & analysed for N-myc downstream-regulated gene 2 (NDRG2) expression by immunoblotting. Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00057447-m03_mouse-monoclonal-ndrg2-antibody-6a5-310202415392511.jpg)
![Western Blot: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 interacts with GLUT1. (A) SK-BR-3 cells were fixed & incubated with primary antibodies against N-myc downstream-regulated gene 2 (NDRG2) or glucose transporter 1 (GLUT1) & with fluorescein isothiocyanate or a cyanine 3 secondary antibody. Green fluorescence indicates NDRG2 expression, red fluorescence indicates GLUT1 expression & blue fluorescence indicates nuclear staining. The results of the merged images reveal that NDRG2 & GLUT1 were colocalised in the cytoplasm. (B) Immunoprecipitation (IP) assays were performed with whole-cell lysates of SK-BR-3 cells pretreated with protein A–conjugated sepharose beads. Whole-cell lysates were probed for input. The antibodies for immunoprecipitation & Western blot (WB) analyses were carried out as indicated. The locations of various proteins are indicated by arrowheads. IgG, Immunoglobulin G. Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00057447-m03_mouse-monoclonal-ndrg2-antibody-6a5-31020241548223.jpg)
![Immunocytochemistry/ Immunofluorescence: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 decreases the glucose uptake & GLUT1 protein levels in SK-BR-3-based subcutaneously xenograft tumours. The experiments illustrated are described in the Methods section. (A) Tumour growth was assessed every 3 days until day 21 treatment by measuring two perpendicular diameters & calculating the volume in cubic centimetres. Ad-LacZ, adenovirus expressing LacZ; Ad-NDRG2, adenovirus expressing NDRG2; PFU, Plaque-forming units. The data presented are means ± SD; error bars represent SD from 6 mice. *P < 0.05 & **P < 0.01 versus phosphate-buffered saline (PBS) or Ad-LacZ. (B) Tumour cells were dissociated from xenograft tumours & suspended in PBS after the number of cells was counted. Next, the glucose uptake of cells in each group was detected. The data presented are means ± SD of three independent experiments; error bars represent SD from 6 mice. *P < 0.05 & **P < 0.01 versus PBS or Ad-LacZ. (C) Intratumoural protein expression was assessed by N-myc downstream-regulated gene 2 (NDRG2) & glucose transporter 1 (GLUT1) IHC staining. Representative images are shown. Original magnification: 400 x; Scale bars = 50 μm. (D) Proteins of the xenograft tumours from each group were extracted & analysed by immunoblotting to quantify NDRG2 & GLUT1 protein changes. Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00057447-m03_mouse-monoclonal-ndrg2-antibody-6a5-310202415482213.jpg)
![Immunohistochemistry: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 is correlated with increased survival & negatively correlated with GLUT1 in breast carcinoma. Kaplan–Meier analysis was carried out according to N-myc downstream-regulated gene 2 (NDRG2) expression levels of disease-free survival (A) & overall survival (B). (C) Serial immunostained sections for NDRG2 & glucose transporter 1 (GLUT1) in breast cancer & normal tissues were analysed. Original magnification, 40× (top) & 400× (bottom); scale bars = 50 μm. (D) Protein was extracted from matched breast tumour tissue (T) & adjacent normal tissue (N) & subjected to immunoblot analysis to examine NDRG2 & GLUT1 expression. beta -actin served as a loading control. P: patient. Relative expression levels of NDRG2 (E) & GLUT1 (F) in human breast cancer & adjacent normal tissue are shown. immunoreactivity score distribution of cancer & adjacent normal tissue were represented with black & brown closed circles, respectively. The horizontal lines presented are means; error bars represented SD from 30 samples. P < 0.01 was considered a statistically significant difference. Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00057447-m03_mouse-monoclonal-ndrg2-antibody-6a5-310202415483635.jpg)
![Immunocytochemistry/ Immunofluorescence: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 interacts with GLUT1. (A) SK-BR-3 cells were fixed & incubated with primary antibodies against N-myc downstream-regulated gene 2 (NDRG2) or glucose transporter 1 (GLUT1) & with fluorescein isothiocyanate or a cyanine 3 secondary antibody. Green fluorescence indicates NDRG2 expression, red fluorescence indicates GLUT1 expression & blue fluorescence indicates nuclear staining. The results of the merged images reveal that NDRG2 & GLUT1 were colocalised in the cytoplasm. (B) Immunoprecipitation (IP) assays were performed with whole-cell lysates of SK-BR-3 cells pretreated with protein A–conjugated sepharose beads. Whole-cell lysates were probed for input. The antibodies for immunoprecipitation & Western blot (WB) analyses were carried out as indicated. The locations of various proteins are indicated by arrowheads. IgG, Immunoglobulin G. Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00057447-m03_mouse-monoclonal-ndrg2-antibody-6a5-310202415483614.jpg)
![Western Blot: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 downregulates GLUT1 by promoting its ubiquitination. (A), (C) & (E) SK-BR-3 cells were infected with an adenovirus carrying N-myc downstream-regulated gene 2 (Ad-NDRG2) at 1, 5 & 10 multiplicity of infection (MOI) or Ad-LacZ for 48 hours. (B), (D) & (F) T-47D cells were transfected with NDRG2 small interfering RNA (siRNA) 10, 25 & 100 pmol or control siRNA for 48 hours. Next, cell proteins or mRNA were extracted & analysed by immunoblotting (A) & (B) or by real-time PCR (C) to (F). beta -actin was used as a loading control. (C) – (F) The data presented are the means ± SD of three independent experiments; error bars represent SD from 3 replicative wells. *P < 0.05 & **P < 0.01 versus control group. (G) SK-BR-3 cells were infected with 10 MOI Ad-NDRG2 or Ad-LacZ for 48 hours & then treated with 2 μM, 6 μM or 8 μM MG-132 for 4 hours. Next, the protein was extracted & analysed by immunoblotting. (H) Cell fractions were prepared from the SK-BR-3 cells infected with 10 MOI Ad-NDRG2 or Ad-LacZ for 48 hours, & the membrane & cytosolic fractions of endogenous glucose transporter 1 (GLUT1) protein were detected. Tubulin & beta -actin served as loading controls. (I) SK-BR-3 cells were transfected with hemagglutinin (HA)-ubiquitin plasmid for 6 hours & infected with Ad-NDRG2 or Ad-LacZ for another 48 hours. Subsequently, the cell lysates were collected & analysed by immunoprecipitation (IP) & immunoblotting with GLUT1 & HA antibodies. WB, Western blot. Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00057447-m03_mouse-monoclonal-ndrg2-antibody-6a5-310202415482275.jpg)
![Western Blot: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 downregulates GLUT1 by promoting its ubiquitination. (A), (C) & (E) SK-BR-3 cells were infected with an adenovirus carrying N-myc downstream-regulated gene 2 (Ad-NDRG2) at 1, 5 & 10 multiplicity of infection (MOI) or Ad-LacZ for 48 hours. (B), (D) & (F) T-47D cells were transfected with NDRG2 small interfering RNA (siRNA) 10, 25 & 100 pmol or control siRNA for 48 hours. Next, cell proteins or mRNA were extracted & analysed by immunoblotting (A) & (B) or by real-time PCR (C) to (F). beta -actin was used as a loading control. (C) – (F) The data presented are the means ± SD of three independent experiments; error bars represent SD from 3 replicative wells. *P < 0.05 & **P < 0.01 versus control group. (G) SK-BR-3 cells were infected with 10 MOI Ad-NDRG2 or Ad-LacZ for 48 hours & then treated with 2 μM, 6 μM or 8 μM MG-132 for 4 hours. Next, the protein was extracted & analysed by immunoblotting. (H) Cell fractions were prepared from the SK-BR-3 cells infected with 10 MOI Ad-NDRG2 or Ad-LacZ for 48 hours, & the membrane & cytosolic fractions of endogenous glucose transporter 1 (GLUT1) protein were detected. Tubulin & beta -actin served as loading controls. (I) SK-BR-3 cells were transfected with hemagglutinin (HA)-ubiquitin plasmid for 6 hours & infected with Ad-NDRG2 or Ad-LacZ for another 48 hours. Subsequently, the cell lysates were collected & analysed by immunoprecipitation (IP) & immunoblotting with GLUT1 & HA antibodies. WB, Western blot. Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00057447-m03_mouse-monoclonal-ndrg2-antibody-6a5-310202415482288.jpg)
![Western Blot: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 decreases the glucose uptake & GLUT1 protein levels in SK-BR-3-based subcutaneously xenograft tumours. The experiments illustrated are described in the Methods section. (A) Tumour growth was assessed every 3 days until day 21 treatment by measuring two perpendicular diameters & calculating the volume in cubic centimetres. Ad-LacZ, adenovirus expressing LacZ; Ad-NDRG2, adenovirus expressing NDRG2; PFU, Plaque-forming units. The data presented are means ± SD; error bars represent SD from 6 mice. *P < 0.05 & **P < 0.01 versus phosphate-buffered saline (PBS) or Ad-LacZ. (B) Tumour cells were dissociated from xenograft tumours & suspended in PBS after the number of cells was counted. Next, the glucose uptake of cells in each group was detected. The data presented are means ± SD of three independent experiments; error bars represent SD from 6 mice. *P < 0.05 & **P < 0.01 versus PBS or Ad-LacZ. (C) Intratumoural protein expression was assessed by N-myc downstream-regulated gene 2 (NDRG2) & glucose transporter 1 (GLUT1) IHC staining. Representative images are shown. Original magnification: 400 x; Scale bars = 50 μm. (D) Proteins of the xenograft tumours from each group were extracted & analysed by immunoblotting to quantify NDRG2 & GLUT1 protein changes. Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00057447-m03_mouse-monoclonal-ndrg2-antibody-6a5-310202415482240.jpg)
![Western Blot: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 inhibits cell proliferation & reduces intracellular glucose levels of breast cancer cells. (B) SK-BR-3 cells with low NDRG2 expression infected by an adenovirus carrying NDRG2 (Ad-NDRG2) or negative control LacZ (Ad-LacZ), & T-47D cells with high NDRG2 transfected with small interfering RNA targeting NDRG2 (NDRG2 siRNA) or negative control siRNA (Con siRNA). Thereafter proteins extracted from these cells & analysed by immunoblotting. beta -actin used as a loading control. Before being cultured in 25 mM high-glucose (H.G.) or 5.5 mM low-glucose (L.G.) medium, SK-BR-3 cells infected by Ad-NDRG2 (C) & T-47D cells transfected by NDRG2 siRNA (D). Cell proliferation was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay for 1 to 5 days.Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00057447-m03_mouse-monoclonal-ndrg2-antibody-6a5-310202415485122.jpg)
![Western Blot: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 is correlated with increased survival & negatively correlated with GLUT1 in breast carcinoma. Kaplan–Meier analysis was carried out according to N-myc downstream-regulated gene 2 (NDRG2) expression levels of disease-free survival (A) & overall survival (B). (C) Serial immunostained sections for NDRG2 & glucose transporter 1 (GLUT1) in breast cancer & normal tissues were analysed. Original magnification, 40× (top) & 400× (bottom); scale bars = 50 μm. (D) Protein was extracted from matched breast tumour tissue (T) & adjacent normal tissue (N) & subjected to immunoblot analysis to examine NDRG2 & GLUT1 expression. beta -actin served as a loading control. P: patient. Relative expression levels of NDRG2 (E) & GLUT1 (F) in human breast cancer & adjacent normal tissue are shown. immunoreactivity score distribution of cancer & adjacent normal tissue were represented with black & brown closed circles, respectively. The horizontal lines presented are means; error bars represented SD from 30 samples. P < 0.01 was considered a statistically significant difference. Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00057447-m03_mouse-monoclonal-ndrg2-antibody-6a5-31020241548518.jpg)
![Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424] - Analysis in human salivary gland and pancreas tissues using NBP1-81424 antibody. Corresponding NDRG2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NDRG2-Antibody-Immunohistochemistry-Paraffin-NBP1-81424-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424] - Analysis in human salivary gland and pancreas tissues using NBP1-81424 antibody. Corresponding NDRG2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: NDRG2 Antibody [NBP1-81424] - Analysis in human liver tissue.](http://images.novusbio.com/fullsize/NDRG2-Antibody-Western-Blot-NBP1-81424-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: NDRG2 Antibody [NBP1-81424] - Staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol and microtubule organizing center. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NDRG2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81424-img0014.jpg)
![Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424] - Staining of human pancreas shows weak cytoplasmic positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/NDRG2-Antibody-Immunohistochemistry-Paraffin-NBP1-81424-img0021.jpg)
![Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424] - Staining of human salivary gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NDRG2-Antibody-Immunohistochemistry-Paraffin-NBP1-81424-img0016.jpg)
![Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424] - Staining of human breast shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NDRG2-Antibody-Immunohistochemistry-Paraffin-NBP1-81424-img0017.jpg)
![Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil and glial cells.](http://images.novusbio.com/fullsize/NDRG2-Antibody-Immunohistochemistry-Paraffin-NBP1-81424-img0018.jpg)
![Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/NDRG2-Antibody-Immunohistochemistry-Paraffin-NBP1-81424-img0019.jpg)
![Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81424] - Staining of human kidney shows moderate cytoplasmic positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/NDRG2-Antibody-Immunohistochemistry-Paraffin-NBP1-81424-img0020.jpg)
![Western Blot: NDRG2 Antibody [NBP1-72052] - (0.1ug/ml) staining of Human Temporal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NDRG2-Antibody-Western-Blot-NBP1-72052-img0002.jpg "Western Blot: NDRG2 Antibody [NBP1-72052] - (0.1ug/ml) staining of Human Temporal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-72052] - 3.75ug/ml staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NDRG2-Antibody-Immunohistochemistry-Paraffin-NBP1-72052-img0004.jpg)
![Western Blot: NDRG2 Antibody [NBP1-72052] - Staining of Mouse (A) and Rat (B) Brain lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NDRG2-Antibody-Western-Blot-NBP1-72052-img0003.jpg)
![Western Blot: NDRG2 Antibody [NBP1-81425] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/NDRG2-Antibody-Western-Blot-NBP1-81425-img0006.jpg "Western Blot: NDRG2 Antibody [NBP1-81425] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81425] - Staining in human cerebral cortex and pancreas tissues using anti-NDRG2 antibody. Corresponding NDRG2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NDRG2-Antibody-Immunohistochemistry-Paraffin-NBP1-81425-img0009.jpg)
![Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81425] - Staining of human salivary gland shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NDRG2-Antibody-Immunohistochemistry-Paraffin-NBP1-81425-img0005.jpg)
![Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81425] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/NDRG2-Antibody-Immunohistochemistry-Paraffin-NBP1-81425-img0007.jpg)
![Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-81425] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/NDRG2-Antibody-Immunohistochemistry-Paraffin-NBP1-81425-img0008.jpg)
![Western Blot: NDRG2 Antibody (4H3) [NBP3-43066] - Various lysates were subjected to SDS PAGE followed by western blot with (NDRG2 Antibody) at dilution of 1:5000.](http://images.novusbio.com/fullsize/nbp3-43066_mouse-ndrg2-mab-4h3-101220241734159.jpg "Western Blot: NDRG2 Antibody (4H3) [NBP3-43066] - Various lysates were subjected to SDS PAGE followed by western blot with (NDRG2 Antibody) at dilution of 1:5000.")
![Immunohistochemistry: NDRG2 Antibody (4H3) [NBP3-43066] - Immunohistochemistry of paraffin-embedded mouse brain using FNab10958 at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-43066_mouse-ndrg2-mab-4h3-101220241734131.jpg)
![Western Blot: NDRG2 Antibody [NBP1-55431] - HepG2 cell lysate, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/NDRG2-Antibody-Western-Blot-NBP1-55431-img0001.jpg "Western Blot: NDRG2 Antibody [NBP1-55431] - HepG2 cell lysate, Antibody Titration: 0.2-1 ug/ml")
![Immunohistochemistry-Paraffin: NDRG2 Antibody [NBP1-55431] - Human Liver tissue, 5 ug/ml.](http://images.novusbio.com/fullsize/NDRG2-Antibody-Immunohistochemistry-Paraffin-NBP1-55431-img0002.jpg)
![Western Blot: NDRG2 Antibody [NBP3-38135] - Western blot analysis of various lysates using NDRG2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/nbp3-38135_rabbit-ndrg2-pab-912202415121375.jpg "Western Blot: NDRG2 Antibody [NBP3-38135] - Western blot analysis of various lysates using NDRG2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.")
![Western Blot: NDRG2 Antibody (1D12) [H00057447-M06] - Analysis of NDRG2 expression in A-431 (Cat # L015V1).](http://images.novusbio.com/fullsize/NDRG2-Antibody-1D12-Western-Blot-H00057447-M06-img0002.jpg "Western Blot: NDRG2 Antibody (1D12) [H00057447-M06] - Analysis of NDRG2 expression in A-431 (Cat # L015V1).")
![Sandwich ELISA: NDRG2 Antibody (1D6) [H00057447-M10] - Detection limit for recombinant GST tagged NDRG2 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NDRG2-Antibody-1D6-Sandwich-ELISA-H00057447-M10-img0001.jpg "Sandwich ELISA: NDRG2 Antibody (1D6) [H00057447-M10] - Detection limit for recombinant GST tagged NDRG2 is 0.1 ng/ml as a capture antibody.")