NDP52 Antibody (OTI4H5) - Azide and BSA Free

Western Blot: NDP52 Antibody (OTI4H5) - Azide and BSA Free [NBP2-72897] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDP52 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDP52.

Flow Cytometry: NDP52 Antibody (OTI4H5) - Azide and BSA Free [NBP2-72897] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NDP52.

Western Blot: NDP52 Antibody (OTI4H5) - Azide and BSA Free [NBP2-72897] - Analysis of extracts (35ug) from 9 different cell lines by using anti-NDP52 monoclonal antibody.

Flow Cytometry: NDP52 Antibody (OTI4H5) - Azide and BSA Free [NBP2-72897] - Analysis of Hela cells, using anti-NDP52 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: NDP52 Antibody (OTI4H5) - Azide and BSA Free [NBP2-72897] - Analysis of Jurkat cells, using anti-NDP52 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: NDP52 Antibody (OTI4H5) - Azide and BSA Free [NBP2-72897] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-NDP52 antibody, and then analyzed by flow cytometry.

• Reactivity: Hu, Pm
• Applications: WB, Flow, ICC/IF, CyTOF-ready
• Clone: OTI4H5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

NDP52 Antibody (OTI4H5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse NDP52 Antibody (OTI4H5) - Azide and BSA Free (NBP2-03246) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human CALCOCO2 (NP_005822) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CALCOCO2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500-2000
• Theoretical MW: 52.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for NDP52 Antibody (OTI4H5) - Azide and BSA Free

• Antigen nuclear dot 52 kDa protein
• calcium binding and coiled-coil domain 2
• calcium-binding and coiled-coil domain-containing protein 2
• NDP52MGC17318
• Nuclear domain 10 protein 52
• Nuclear domain 10 protein NDP52
• Nuclear dot protein 52

Background

NDP52 is encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CALCOCO2