NCOR1 Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat NCOR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6167) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NCOR1 at approximately 270 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

NCOR1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human NCOR1
  Gln1770-Ala1947
  Accession # O75376
• Specificity: Detects human, mouse, and rat NCOR1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NCOR1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NCOR1 Antibody [Unconjugated]

• hN-CoR
• KIAA1047
• KIAA1047N-CoR1
• MGC104216
• N-CoR
• NCOR1
• N-CoRhCIT529I10
• nuclear receptor corepressor 1
• nuclear receptor co-repressor 1
• thyroid hormone- and retinoic acid receptor-associated corepressor 1
• TRAC1

Background

NCOR1 (Nuclear receptor Co-Repressor 1) is a 270 kDa member of the NCoR family of molecules. It is widely expressed, being found in hepatocytes, intestinal crypt cells, neural stem cells, plus immature thymocytes and erythrocytes. NCOR1 is a transcriptional repressor. It forms a complex with HDAC3, TAB2 and ZBTB33, and interacts with a ligand-independent THR:RXR heterodimer bound to select gene promoters. Human NCOR1 is 2440 amino acids (aa) in length. It possesses one N-terminal repression domain (aa 1-312), two DNA-binding SANT domains (aa 437-674) and a second repression domain (aa 737-1004). Multiple Ser, Thr and Tyr phosphorylation sites exist that regulate complex dissociation. There are multiple potential splice variants. Short poly Lys motifs serve as substitutions for the C-terminal 1900-1910 amino acids. There is also a 16 aa insertion after Glu727, coupled to either a Ile substitution for aa 1842-1961, or a six aa substitution for aa 31-145. Over aa 1770-1947, human NCOR1 shares 96% aa identity with mouse NCOR1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCOR1
• Uniprot:
  • O75376(Human)