NCOR1 Antibody [Alexa Fluor® 700]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 700

NCOR1 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human NCOR1
  Gln1770-Ala1947
  Accession # O75376
• Specificity: Detects human, mouse, and rat NCOR1 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NCOR1 Antibody [Alexa Fluor® 700]

• hN-CoR
• KIAA1047
• KIAA1047N-CoR1
• MGC104216
• N-CoR
• NCOR1
• N-CoRhCIT529I10
• nuclear receptor corepressor 1
• nuclear receptor co-repressor 1
• thyroid hormone- and retinoic acid receptor-associated corepressor 1
• TRAC1

Background

NCOR1 (Nuclear receptor Co-Repressor 1) is a 270 kDa member of the NCoR family of molecules. It is widely expressed, being found in hepatocytes, intestinal crypt cells, neural stem cells, plus immature thymocytes and erythrocytes. NCOR1 is a transcriptional repressor. It forms a complex with HDAC3, TAB2 and ZBTB33, and interacts with a ligand‑independent THR:RXR heterodimer bound to select gene promoters. Human NCOR1 is 2440 amino acids (aa) in length. It possesses one N‑terminal repression domain (aa 1‑312), two DNA‑binding SANT domains (aa 437‑674) and a second repression domain (aa 737‑1004). Multiple Ser, Thr and Tyr phosphorylation sites exist that regulate complex dissociation. There are multiple potential splice variants. Short poly Lys motifs serve as substitutions for the C‑terminal 1900‑1910 amino acids. There is also a 16 aa insertion after Glu727, coupled to either a Ile substitution for aa 1842‑1961, or a six aa substitution for aa 31‑145. Over aa 1770‑1947, human NCOR1 shares 96% aa identity with mouse NCOR1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.