NCOA7 Antibody (5Q2U7)

Western Blot: NCOA7 Antibody (5Q2U7) [NBP3-33462] - Western blot analysis of lysates from Mouse brain, using NCOA7 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 5Q2U7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

NCOA7 Antibody (5Q2U7) Summary

• Description: Novus Biologicals Rabbit NCOA7 Antibody (5Q2U7) (NBP3-33462) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 777-942 of human NCOA7.3).
  
  Sequence:
  LRPHSALLENMHIEQLARRLPARVQGYPWRLAYSTLEHGTSLKTLYRKSASLDSPVLLVIKDMDNQIFGAYATHPFKFSDHYYGTGETFLYTFSPHFKVFKWSGENSYFINGDISSLELGGGGGRFGLWLDADLYHGRSNSCSTFNNDILSKKEDFIVQDLEVWAFD
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NCOA7
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for NCOA7 Antibody (5Q2U7)

• 140 kDa estrogen receptor-associated protein
• dJ187J11.3
• ERAP140Nbla00052
• ESNA1
• Estrogen nuclear receptor coactivator 1
• estrogen receptor associated protein 140 kDa
• FLJ45605
• MGC88425
• Nbla10993
• nuclear receptor coactivator 7
• putative protein product of Nbla00052
• putative protein product of Nbla10993

Background

Enhances the transcriptional activities of several nuclear receptors. Involved in the coactivation ofdifferent nuclear receptors, such as ESR1, THRB, PPARG and RARA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCOA7