NCOA2 Antibody [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 700

NCOA2 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human NCOA2
  Phe1090-Leu1247
  Accession # Q15596
• Specificity: Detects human NCOA2 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) NCOA1 is observed, and less than 1% cross-reactivity with rhNCOA4 and rhNCOA6 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NCOA2 Antibody [Alexa Fluor® 700]

• bHLHe75
• bHLHe75MGC138808
• Class E basic helix-loop-helix protein 75
• EC 2.3.1.48
• GRIP1glucocorticoid receptor-interacting protein-1
• HTIF2
• KAT13C
• NCOA2
• NCoA-2SRC2
• nuclear receptor coactivator 2
• TIF2
• TIF2hTIF2
• Transcriptional intermediary factor 2

Background

NCOA2 (Nuclear receptor COActivator 2; also TIF2 and GRIP-1) is a 150-160 kDa, ubiquitously expressed, nuclear member of the SRC/p160 nuclear receptor (NR) coactivator family of proteins. As noted, and although NCOA2 is a coactivator of hormone nuclear receptors, it also contributes to HIF-1 alpha :Arnt driven gene transcription. With respect to NRs, it not only has intrinsic activity that directly impacts hormone receptor gene activation, but also recruits additional coactivators to the NR:p160/NCOA2 complex, imparting additional complexity to target gene expression. NCOA2 binds directly to nuclear receptors via one or more LxxLL motifs that constitute an NID (NR Interaction Domain). It further uses its C-terminus to both recruit additional coactivators such as CBP and CARM1, and to modify key chromatin sites via an intrinsic HAT activity. Human NCOA2 is 1464 amino acids (aa) in length. It contains bHLH domain (aa 26-83), a PAS region (aa 119-183), four consecutive LxxLL motifs over aa 641-882, and a C-terminal LLxxLxxxL segment (aa 1079-1087). There are five utilized Ser/Thr phosphorylation sites, and three utilized acetylation sites on Lys. NCOA2 is known to undergo translocation and fuse with KAT6A/MOZ, creating a fusion product containing aa 870-1464 of NCOA2. Over aa 1090-1247, human NCOA2 shares 94% aa sequence identity with mouse NCOA2/GRIP-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.