NCAPG2 Antibody

Immunocytochemistry/Immunofluorescence: NCAPG2 Antibody [NBP1-86633] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: NCAPG2 Antibody [NBP1-86633] - Staining of human tonsil shows moderate nuclear positivity in germinal center and non-germinal center cells.

Immunohistochemistry-Paraffin: NCAPG2 Antibody [NBP1-86633] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts and Leydig cells.

Immunohistochemistry-Paraffin: NCAPG2 Antibody [NBP1-86633] - Staining of human prostate shows moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: NCAPG2 Antibody [NBP1-86633] - Staining of human skeletal muscle shows weak to moderate nuclear positivity in myocytes.

• Reactivity: Hu
• Applications: ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NCAPG2 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: QVGHILELVDNWLPTEHAQAKSNTASKGRVQIHDTRPVKPELALVYIEYLLTHPKNRECLLSAPRKKLNHLLKALETSKADLESLLQTPGGKPRGFSEAAAPRAFGLHCRLSIHLQH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCAPG2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
• Theoretical MW: 131 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  NCAPG2 Protein (NBP1-86633PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NCAPG2 Antibody

• Chromosome-associated protein G2
• FLJ20311
• Leucine zipper protein 5CAP-G2hCAP-G2condensin-2 complex subunit G2
• LUZP5
• more than blood homolog
• MTB
• Non-SMC condensin II complex subunit G2
• non-SMC condensin II complex, subunit G2

Background

Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 (MIM 605576) and SMC4 (MIM 605575), but they contain different sets of non-SMC subunits. NCAPG2 is 1 of 3 non-SMC subunits that define condensin II (Ono et al., 2003 [PubMed 14532007]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCAPG2
• Entrez:
  • 54892(Human)
• Uniprot:
  • Q86XI2(Human)