NCAM-1/CD56 Antibody (SPM128)

Images

 
Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349] - Formalin-fixed, paraffin-embedded human Pancreas stained with NCAM-1/CD56 Antibody (SPM128)
Flow Cytometry: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349] - FACS analysis of CD56 on human Monocytes using NCAM-1/CD56 Antibody (SPM128)
Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349] - Formalin-fixed, paraffin-embedded human Cerebellum stained with CD56 Monoclonal Antibody (SPM128)
Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349] - Formalin-paraffin human colon ganglion stained with CD56 MAb (SPM128)
Flow Cytometry: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349] - A surface stain was performed on SH-SY5Y cells with NCAM-1/CD56 (SPM128) antibody NBP2-34397APC (blue) and a matched isotype control NBP2-27287APC (orange). ...read more
Flow (Cell Surface): NCAM-1/CD56 Antibody (SPM128) [NBP2-34349] - Flow Cytometry: NCAM-1/CD56 Antibody (SPM128) - Azide and BSA Free [NBP2-34397] - A surface stain was performed on SH-SY5Y cells with NCAM-1/CD56 ...read more
Flow Cytometry: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349] - A surface stain was performed on U-87 MG cells with NCAM-1/CD56 Antibody (SPM128) NBP2-34397AF647 (blue) and a matched isotype control (orange). Cells were ...read more

Product Details

Summary
Reactivity Hu, Rt, ZeSpecies Glossary
Applications WB, Flow, Flow-CS, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
SPM128
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

NCAM-1/CD56 Antibody (SPM128) Summary

Immunogen
Membrane preparation of a small cell lung carcinoma
Localization
Cell surface
Marker
Neuronal Cell Marker
Specificity
This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NCAM1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 1-2ug/500ug
  • Flow (Cell Surface)
Application Notes
ELISA (For coating, order NBP2-34397 (BSA free version of NBP2-34349)). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Control
NCAM-1/CD56 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for NCAM-1/CD56 Antibody (SPM128)

  • CD56 / NCAM-1
  • CD56 antigen
  • CD56
  • MSK39
  • NCAM1
  • N-CAM-1
  • NCAM-1
  • NCAMantigen recognized by monoclonal 5.1H11
  • neural cell adhesion molecule 1
  • neural cell adhesion molecule, NCAM

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NCAM-1/CD56 Antibody (NBP2-34349) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34397AF350
Alexa Fluor 405 NBP2-34397AF405
Alexa Fluor 488 NBP2-34397AF488
Alexa Fluor 532 NBP2-34397AF532
Alexa Fluor 594 NBP2-34397AF594
Alexa Fluor 647 NBP2-34397AF647
Alexa Fluor 700 NBP2-34397AF700
Alexa Fluor 750 NBP2-34397AF750
Allophycocyanin NBP2-34397APC
Biotin NBP2-34397B
DyLight 350 NBP2-34397UV
DyLight 405 NBP2-34397V
DyLight 488 NBP2-34397G
DyLight 550 NBP2-34397R
DyLight 594 NBP2-34397DL594
DyLight 650 NBP2-34397C
DyLight 680 NBP2-34397FR
DyLight 755 NBP2-34397IR
FITC NBP2-34397F
HRP NBP2-34397H
Janelia Fluor 549 NBP2-34397JF549
Janelia Fluor 646 NBP2-34397JF646
PE NBP2-34397PE
PerCP NBP2-34397PCP

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Bioinformatics

Gene Symbol NCAM1