NCAM-1/CD56 Antibody (MEM-188) - BSA Free

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Flow Cytometry: NCAM-1/CD56 Antibody (MEM-188) [NBP3-09126] - Analysis (surface staining) of human peripheral blood lymphocytes with anti-CD56 (MEM-188) purified; GAM-APC.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications Flow, ICC/IF, IHC, IP
Clone
MEM-188
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

NCAM-1/CD56 Antibody (MEM-188) - BSA Free Summary

Additional Information
Clone MEM-188 was used by HLDA to establish CD designation.
Immunogen
KG-1 human acute myelogenous leukemia cell line
Specificity
The antibody MEM-188 reacts with an extracellular epitope on a 180 kDa isoform of CD56 (NCAM) expressed in leukocytes. It has been suggested that the antibody MEM-188 could react with rhesus monkey lymphocytes. Reactivity with other NCAM isoforms has not been tested.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NCAM1
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1 ug/ml
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for NCAM-1/CD56 Antibody (MEM-188) - BSA Free

  • CD56 / NCAM-1
  • CD56 antigen
  • CD56
  • MSK39
  • NCAM1
  • N-CAM-1
  • NCAM-1
  • NCAMantigen recognized by monoclonal 5.1H11
  • neural cell adhesion molecule 1
  • neural cell adhesion molecule, NCAM

Background

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NCAM1